Sequences from confirmation amplicon


Confirmation sequences for line 602D02 and hit At3g57240 are shown.
Line ID 602D02
Locus At3g57240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21182333
Primers used for PCR 50jx - 8474, show primer details
   
Sequence Name 80-K113379-0022-602-D02C-50jx
GenBank Accession LN513969 [GenBank]
Sequencing primer gene specific primer, 50jx: 5'-GAATTTTAAGTTGGACAAATCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113379-0022-602-D02C-50jx
GACCCGCGTCCGAGTCGTGGCGCTTTACCACAACGGAACATCCGGCGGATGCGGCTGTAC
GATCCCAACCAGGAGACTCTCAACGCTCTCCGCGGCTCCAATATCGAGCTCGTGCTCGAT
GTTCCAAACCCTGATCTCCAGCGTCTCGCCTCCAGCCAAGCGGAAGCCGACACATGGGTC
CGCAACAACGTCCGCAACTATGCAAACGTCACATTTCGCTACATCTCTGTCGGGAACGAG
GTACAGCCCTCAGACCAAGCTGCGAGCTTTGTCCTCCCCGCGATGCAGAACATAGAGAGA
GCAGTTTCAAGCCTCGGGATCAAGGTCTCGACGGCTATAGACACCAGAGGCATTTCCGGG
TTTCCGCCGTCAAGTGGGACGTTCACACCGGAGTTTAGGAGCTTTATCGCACCGGTGATA
AGTTTCTTGTCAAGCAAGCAATCTCCTCTGCTCGTGAATAATTACCCTTACTTCAGCTAC
ACGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 42-K113377-0022-602-D02C-8409
GenBank Accession LN504530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113377-0022-602-D02C-8409
TAGGGTATTATTCACGAGCAGAGGAGATTGCTTGCTTGACAGAAACTTATCACCGGTGCG
ATAAAGCTCCTAAACTCCGGTGTGAACGTCCCACTTGACGGCGGAAACCCGGAAATGCCT
CTGGTGTCTATAGCCGTCGAGACCTTGATCCCGAGGCTTGAAACTGCTCTCTCTATGTTC
TGCATCGCGGGGAGGACAAAGCTCGCAGCTTGGTCTGAGGGCTGTACCTCGTTCCCGACA
GAGATGTAGCGAAATGTGACGTTTGCATAGTTGCGGACGTTGTTGCGGACCCATGTGTCG
GCTTCCGCTTGGCTGGAGGCGAGACGCTGGAGATCAGGGTTTGGAACATCGAGCACGAGC
TCGATATTGGAGCCGCGGAGAGCGTTGAGAGTCTCCTGGTTGGGATCGTACAGCCGCATC
CGCCGGATGTTCCGTTGTTGGTAAAGCGCCACGACTTCGGACGCGGGTCGCAGGTTGTTT
CCATTTCTCCCGTAGCACACTCCGATTTGTCCAACTTAAAAT

Last Updated on 06.12.2018 16:11