Sequences from confirmation amplicon


Confirmation sequences for line 603A06 and hit At5g09930 are shown.
Line ID 603A06
Locus At5g09930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3098407
Primers used for PCR BA28 - 8474, show primer details
   
Sequence Name 32-K104830-0022-603-A06C-BA28
GenBank Accession LN500575 [GenBank]
Sequencing primer gene specific primer, BA28: 5'-TTGATCTGTATTTCTGATGCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104830-0022-603-A06C-BA28
AAAGAGAGAGAAGTCGCTATAATTGGTCCAACGGGTGCGGAAAGAGTACATTGCTGAAAC
TTATTATGGGTTTGGAGAAGCCTATGAGAGGTGAGGTGATACTTGGGGAGCACAATGTAT
TGCCAAACTACTTCGAGCAGAATCAGGTTTGTTCTATCATGAAGAAATGGCTTTTTAGAG
GAATCAGAAAATACAGTTTTACTCATTTGTTTGGCTGTAGGCAGAGGCTCAAGATTTGGA
TAAAACGGTGATTGAGACAGTTGTTGAAGCTGCTGCTATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 47-K104831-0022-603-A06C-8409
GenBank Accession LN506196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K104831-0022-603-A06C-8409
TTACAATTGAATAGCAGCAGCTTCAACAACTGTCTCAATCACCGTTTTATCCAAATCTTG
AGCCTCTGCCTACAGCCAAACAAATGAGTAAAACTGTATTTTCTGATTCCTCTAAAAAGC
CATTTCTTCATGATAGAACAAACCTGATTCTGCTCGAAGTAGTTTGGCAATACATTGTGC
TCCCCAAGTATCACCTCACCTCTCATAGGCTTCTCCAAACCCATAATAAGTTTCAGCAAT
GTACTCTTTCCGCACCCGTTTGGACCAATTATAGCGACTTTCTCTCCTCTTTCTATAGCT
AGATTCGCCTTGTTAAATAACATCTGCTTGCATCAGAAATACAGATCA
Primers used for PCR BA29 - 3144, show primer details
   
Sequence Name 33-K104830-0022-603-A06C-BA29
GenBank Accession LN500986 [GenBank]
Sequencing primer gene specific primer, BA29: 5'-GCATAGTCCATTAAACCACCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104830-0022-603-A06C-BA29
ATCTGTTTATGAAGTATCTATCGTGTGAGACTGTGATGACCGTGCCTTTGTACTCATTTA
TAGCTTCCTGATGAAAGAAGGAAACAGAGAATCAGATCATACTCAACCTCCAAGAACAGT
TTGGCCTATTGAACAGATAAGAGTATGGTTAGAGACCTCAAGCATTTCTTTTGAAGGTAT
GTCTAAGTGATTGGTGGGTTCGTCCAACACAAGTAGAGTGGATGGTTTCACCATGAATTT
GCAGAAAGCAAGGCGTGCCTAAGACCGCAAGAAAAAAGGCGATGAAGAACATGATGAGTA
TTATATTGAGGATAATGATAGACATTATGTTACCTTCTCACCACCACTCAAAAGAGAGAC
CTTTCTATCCAACATGTCAGCTTTGAAGTTGCACCGACCAACATATCAAACACTGATAGT
TTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAA
ATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCC
TTACGTCAGTGGAGATATCACACCAATCCA
   
Sequence Name 48-K104831-0022-603-A06C-3144
GenBank Accession LN506502 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K104831-0022-603-A06C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATATGTTGGT
CGGTGCAACTTCAAAGCTGACATGTTGGATAGAAAGGTCTCTCTTTTGAGTGGTGGTGAG
AAGGTAACATAATGTCTATCATTATCCTCAATATAATACTCATCATGTTCTTCATCGCCT
TTTTTCTTGCGGTCTTAGGCACGCCTTGCTTTCTGCAAATTCATGGTGAAACCATCCACT
CTACTTGTGTTGGACGAACCCACCAATCACTTAGACATACCTTCAAAAGAAATGCTTGAG
GTCTCTAACCATACTCTTATCTGTTCAATAGGCCAAACTGTTCTTGGAGGTTGAGTATGA
TCTGATTCTCTGTTTCCTTCTTTCATCAGGAAGCTATAAATGAGTACAAAGGCACGGTCA
TCACAGTCTCACACGATAGATACTTCATTAAACAGATTGTTAACAGAGTTATTGAAGTCA
GAGATGGTGGTTTATGGNACTATG

Last Updated on 06.12.2018 16:11