Sequences from confirmation amplicon


Confirmation sequences for line 604B06 and hit At5g01810 are shown.
Line ID 604B06
Locus At5g01810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:310704
Primers used for PCR KS56 - 8474, show primer details
   
Sequence Name 53-K101502-0022-604-B06C-8409
GenBank Accession LN508096 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101502-0022-604-B06C-8409
GCCATTTCCAATCTAAAATCTACTTCGTCATGGAACATGTTAAGGGTGGTGAGCTCTTCA
CAAAGTGTCAACTGGGAAGCTGAGAGAAGACGTTGCGAGAAAGTACTTTCAGCAGCTTGT
ACGCGCTGTTGACTTCTGTCACAGCCGTGGAGTATGCCACAGGGACCTGAAGCCGGAGAA
TCTCTTGTTGGATGAGCATGGGAATCTTAAGATCTCTGATTTTGGTCTCAGCGCTCTTTC
TGACTCTAGAAGGCAAGACGGGTTGCTGCATACTACATGCGGGACCCCTGCATATGTTGC
ACCGGAGGTGATAAGCAGGAACGGGTATGATGGGTTTAAAGCGGATGTGTGGTCCTGTGG
AGTGATATTGTTCGTCTTGCTCGCTGGATATCTTCCTTTCCGTGATTCCNATCTGATGGA
GCTGTATAAGAAGATAGGCAAAGCTGAAGTCAAGTTCCCCAACTGGCTTGCTCCGGGGGC
AAAGAGATTGCTCAAGAGGATCTTGGATCCTAACCCCAACACAAGGGTATC
   
Sequence Name 53-K101506-0022-604-B06C-KS56
GenBank Accession LN508097 [GenBank]
Sequencing primer gene specific primer, KS56: 5'-TTCTCAGTTGATACCCTTGTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101506-0022-604-B06C-KS56
ATCTCTTTGCCCCGGAGCAGCCAGTTGGGGAACTTGACTTCAGCTTTGCCTATCTTCTTA
TACAGCTCCATCAGATTGGAATCACGGAAAGGAAGATATCCAGCGAGCAAGACGAACAAT
ATCACTCCACAGGACCACACATCCGCTTTAAACCCATCATACCCGTTCCTGCTTATCACC
TCCGGTGCAACATATGCAGGGGTCCCGCATGTAGTATGCAGCAACCCGTCTTGCCTTCTA
GAGTCAGAAAGAGCGCTGAGACCAAAATCAGAGATCTTAAGATTCCCATGCTCATCCAAC
AAGAGATTCTCCGGCTTCAGGTCCCTGTGGCATACTCCACGGCTGTGACAGAAGTCAACA
GCGCGTACAAGCTGCTGAAAGTACTTTCTCGCAACGTCTTCTCTCAGCTTCCCAGTTGAC
ACTTTGTTGAAGAGCTCACCACCCTTAACATGTTCCATGA

Last Updated on 06.12.2018 16:11