Sequences from confirmation amplicon


Confirmation sequences for line 604C03 and hit At3g53240 are shown.
Line ID 604C03
Locus At3g53240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19738593
Primers used for PCR DR61 - 8474, show primer details
   
Sequence Name 34-K035537-022-604-C03C-8409
GenBank Accession LN501323 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K035537-022-604-C03C-8409
GCCTTTAAATTGAACCCGATAATGAATTGCGTGAGAGATTCAGCGAGCGTACTCTCTTAA
GATCTCCTAGCTCTTCTGGGATATTACCACTTAACTCGTTGCTCGACAGATCCAGGCCAA
ACATCTGGTTAAGAGTTCCTCTCATGTACAAGTCATATCTCTGTTTCACCGCAAATTCAA
CTTGGACATTAAAATCTACCGAGTAGTCTAGAGAAAACCGATCTGACACAATCAAAGACT
CGTAATATACTTCGGTGTATATCTCCATAAAGTTTGAGAGCAAGCTCGCCGGATACCAAT
CTGAATCAGCATTACTATGTCCTCCTGAGCCAAATGATAGATTGGTGACACACGAAGGTA
TGGATTCATTCAATCGATTGTGAGCAAAATCCAACATCCTTACGTTGCTTAAACCGCACA
GCTCAACAGGAATCTTTCCTGTCAAGTTATTCTCTCTCAACAGAACAACACTGATGCTCG
GGGTGCTCCTGAACAGTGGAATGTTTCCAGACAGTTTGTTGTTTCTCAGATCAAGCAACC
GTAGCCCGTACCACAACGTGTCTGGAATAGATCCAGTGAGATGGTTGTTGTG
   
Sequence Name 82-K035537-022-604-C03C-DR61
GenBank Accession LN514144 [GenBank]
Sequencing primer gene specific primer, DR61: 5'-CACAACAACAATCTCACTGGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K035537-022-604-C03C-DR61
TTGCTTGATCTGAGAAACAACAAACTGTCTGGAAACATTCCACTGTTCAGGAGCACCCCG
AGCATCAGTGTTGTTCTGTTGAGAGAGAATAACTTGACAGGAAAGATTCCTGTTGAGCTG
TGCGGTTTAAGCAACGTAAGGATGTTGGATTTTGCTCACAATCGATTGAATGAATCCATA
CCTTCGTGTGTCACCAATCTATCATTTGGCTCAGGAGGACATAGTAATGCTGATTCAGAT
TGGTATCCGGCGAGCTTGCTCTCAAACTTTATGGAGATATACACCGAAGTATATTACGAG
TCTTTGATTGTGTCAGATCGGTTTTCTCTAGACTACTCGGTAGATTTTAATGTCCAAGTT
GAATTTGCGGTGAAACAGAGATATGACTTGTACATGAGAGGAACTCTTAACCAGATGTTT
GGCCTGGATCTGTCGAGCAACGAGTTAAGTGGTAATATCCCAGAAGAGCTAGGAGATCTT
AAGAGAGTACGCTCGCTGAATCTCTCACGCAATTCATTATCGGGTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCC
CAAATGG

Last Updated on 06.12.2018 16:11