Sequences from confirmation amplicon


Confirmation sequences for line 604C11 and hit At1g07450 are shown.
Line ID 604C11
Locus At1g07450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2288534
Primers used for PCR 41sa - 8474, show primer details
   
Sequence Name 61-K116229-0022-604-C11C-41sa
GenBank Accession LN510602 [GenBank]
Sequencing primer gene specific primer, 41sa: 5'-GGTTGAGCTTGACTTTAGGTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K116229-0022-604-C11C-41sa
TTTCCCTCTCGACCTCAGAGAGTGCAATTGATGCAGACTGTTTCTTCTCTATTTGGTGCC
AAACTCAACATCCTTGTAAGGTCTTCTTCAAGCTTTCCTAGGTTCTTATGGTCTCATTTG
GGTCTTGATTGGTTTTTGTAAAGCAAAAAAAAAAAGGAATAAATGGGCTTTTGGAATACC
TTAATAACGTCTTTGTTTTGCCTTTATGCAAATAAACAATGTTGGCAATTACTTTTTAAA
CCCAATTTTAAAAATTACACCTAAAAATTTCTCAATTTTAATGGCTACCATTTTGAAATC
TGCTTACTACTTCTCCCATTTGGCACTTCCTTTACTTAAACCCTCGGAAAATGGAAATAT
CGTGTTTTTTTCCTCGGTAACGGGGTTTGTCATACAATATCGTGTATTTTCATTTGTAAA
TGGCTTCTTGTCCGGAAATTCTACTTGGATCACCATTGATTATGATGGTCAATATGGAAA
AAAAAAAAGATTATTTCCCATTTTTTTTTCATTTCAAAATTGTA
   
Sequence Name 21-K116228-0022-604-C11C-8409
GenBank Accession LN495809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116228-0022-604-C11C-8409
GCTTTAAGTAAAGGAAGCGCCAATTGGGAGAAGTAGTAAGCAGATTTCAAATTGGTAGCC
ATTAAACTTGAGAAATCTTCAGCTGTACTTTCTAAAGTTGGCTTTAATATGTACTTGCCA
ACATTGTTTATCTGCATAATGGCAAACCAATGACGTTATTAAGGTATCCCAAACGCCCAT
TTATCCTTTTTTTTTTTGTTTTACAAAACCCATTCAAAACCCAATTGAAACCTTAAAACC
CTAGAAAACTTTGAAAAAAACTTTACAAGGATTTTAATTTTGCCCCCAAATAAAAAAAAA
ACATTCTGCATCATTTGCACTCTCTAAGGTCAAAAGAAAACTTCACAAATTGAACCCCTC
ATTTCAAACCTTTTTGCATGCCATCCATTTAACCTTTCTTTTACCAAATTTTCTTCTATT
TCACAAATTTAAACTCTTGCACCAAACCCTACAATTTCCTCCACTATGGCATCCCTA

Last Updated on 06.12.2018 16:11