Sequences from confirmation amplicon


Confirmation sequences for line 605A03 and hit At5g62250 are shown.
Line ID 605A03
Locus At5g62250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25006501
Primers used for PCR JV22 - 8474, show primer details
   
Sequence Name 67-K032929-022-605-A03C-8409
GenBank Accession LN511738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K032929-022-605-A03C-8409
CATTTACAATTGAATAGGGAAGAATTTACATTGAAGAACTCTTGAATCGCTTTGTTCGGT
TCTTGGTTTGAATTTTCGAGAAACTGTTACCAAGATTCACCCAAGTTTAGTAGATACTGA
AGGGTCAAGAAGTATAAGTAATGAAACACTTGACAAGTTAGCTTCATCAGTACAACAATG
GCATGAGACAAAGATTCAAAGAATGCAAGAAGTGAGTTAGAATGTTTTTAAACATTTTAC
CATCTTAAAAACTCTGCTCTTTGTTTCTTATATATATATGCTATCTTATGGATTACAGCT
TCAAGATCTCGTGACAACGATGCTTGAGTTTTGGAATTTAATGGATACACCAGCAGAAGA
ACAACAAAAGTTCATGGATGTATCATGTAATATAGCTGCTACTGTTTCTGAAATAACCAA
ACCCAATAGTCTTTCTATAGATTTGCTAGAAGAGGTAACTTCACAACAACTTTACATCAA
ATCTTTTTGTAAACTTACTGAGACTATTTTCGCCGATGGATTGCTTAATGTTTTAAATAG
GTTAAAGCTGAGCTATGTCGGTTGGAGGAGTTGAAGTGGAGCAAATG
   
Sequence Name 67-K032929-022-605-A03C-JV22
GenBank Accession LN511739 [GenBank]
Sequencing primer gene specific primer, JV22: 5'-GAACAAGTTCTTTCATTTTGCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K032929-022-605-A03C-JV22
ACTCAGCTTTACCTATTTAAACATTAAGCAATCCATCGGCGAAAATAGTCTCAGTAAGTT
TACAAAAAGATTTGATGTAAAGTTGTTGTGAAGTTACCTCTTCTAGCAAATCTATAGAAA
GACTATTGGGTTTGGTTATTTCAGAAACAGTAGCAGCTATATTACATGATACATCCATGA
ACTTTTGTTGTTCTTCTGCTGGTGTATCCATTAAATTCCAAAACTCAAGCATCGTTGTCA
CGAGATCTTGAAGCTGTAATCCATAAGATAGCATATATATATAAGAAACAAAGAGCAGAG
TTTTTAAGATGGTAAAATGTTTAAAAACATTCTAACTCACTTCTTGCATTCTTTGAATCT
TTGTCTCATGCCATTGTTGTACTGATGAAGCTAACTTGTCAAGTGTTTCATTACTTATAC
TTCTTGACCCTTCAGTATCTACTAAACTTGGGTGAATCTTGGTAACAGTTTCTCGAAAAT
TCAAACCAAGAACCGAACAAAGCGATTCAAGAGTTCTTCAATGTAAATTTCTTCCCTATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGT
CTAC

Last Updated on 06.12.2018 16:11