Sequences from confirmation amplicon


Confirmation sequences for line 605F08 and hit At3g53390 are shown.
Line ID 605F08
Locus At3g53390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19793517
Primers used for PCR 29et - 8474, show primer details
   
Sequence Name 28-K110678-0022-605-F08C-29et
GenBank Accession LN498897 [GenBank]
Sequencing primer gene specific primer, 29et: 5'-CATGGCAAAACTGTTACTCAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K110678-0022-605-F08C-29et
TTGGGTTTATAGTTTGATTCGTCATTAGGATTTCTAATCATTCAAATCGGCAATTTCAGA
GAATTAGTATCGATGGTTAAATTTTTGTTAGTGAAATTTTGCATTTTGATGCACTAGTTG
GTTAGCTGATCAATCGATTTGAATAGCATTGCTTTCCAATGATGGAATTTGGAAATTTTC
GTTTTCAGGTAACTATAGCTCAGCTTAAGGAAAGAGCTGCTCCTTCGACTCCAACTGGTA
CTTCGTCGGGTTATAGTGCTAGTAGCGGGTTTCGTTCAGCAACAGCGAGGTTGCTAGGAA
CTGGAAATGGGAGCCGTGCGCTTAGTTTTGTTGGAGGTAATGGTGGCGGTAAAAGTGTGA
GTACAAGTTCAAGAATTAGTGGCTCCTTCGCTGCTTCTAATTCTAATACTTCGATGACAA
ATACGAATTTTGATGGGAAAGGAACTTACTTGGTCTTCAATGTGAGTGATGCTATTTTTA
TCTGTGACTTGAACTCACAAGAAAAGGTAATTTCTTCTTTTCTTTGTTTTCCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
GTCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGT
   
Sequence Name 12-K110677-0022-605-F08C-8409
GenBank Accession LN491630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K110677-0022-605-F08C-8409
CGGACTGAGCCTTTACATTGAATATATCCTGGAAAACAAAGAAAAGAAGAAATTACCTTT
TCTTGTGAGTTCAAGTCACAGATAAAAATAGCATCACTCACATTGAAGACCAAGTAAGTT
CCTTTCCCATCAAAATTCGTATTTGTCATCGAAGTATTAGAATTAGAAGCAGCGAAGGAG
CCACTAATTCTTGAACTTGTACTCACACTTTTACCGCCACCATTACCTCCAACAAAACTA
AGCGCACGGCTCCCATTTCCAGTTCCTAGCAACCTCGCTGTTGCTGAACGAAACCCGCTA
CTAGCACTATAACCCGACGAAGTACCAGTTGGAGTCGAAGGAGCAGCTCTTTCCTTAAGC
TGAGCTATAGTTACCTGAAAACGAANATTTCCAAATTCCATCATTGGAAAGCAATGCTAT
TCAAATCGATTGATCAGCTAACCAACTAGTGCATCAAAATGCAAAATTTCACTAACAAAA
ATTTACCATCGATACTAATTCTCTGAAATTGCGATTTGAATGAATAGAATCCTAATGACG
AATCAAACTATTAAACCCAAATATAAAATTTGATCTT

Last Updated on 06.12.2018 16:11