Sequences from confirmation amplicon


Confirmation sequences for line 606E07 and hit At4g08960 are shown.
Line ID 606E07
Locus At4g08960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5746238
Primers used for PCR NL02 - 8474, show primer details
   
Sequence Name 80-K103929-0022-606-E07C-NL02
GenBank Accession LN513945 [GenBank]
Sequencing primer gene specific primer, NL02: 5'-CCTTAACGGCAAATAAGATTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K103929-0022-606-E07C-NL02
ACTTCGTTATGTCCGAGCTATACGGTTACTTTGACTTTGAGGCATCACTACCTTGAACTT
ATACACCACTAGAAACTTCGAACTAGGTTGTGTATATGGCCAAAGTATGTCCGAGAACGG
GCGCAGGGTTATTCAGAGTTTCAATAGATCATGTCAGCTTCGAAAGAATCCACAAAATAT
AACACAATATTGCCGTAATCTTGTTAAGGGTATGTAGAAAAGCATCGTAGCCCACGCAGC
AACACCACATACAATAAAACTTGAATTAGTCCATGAAGAACTCACTATTTACATTCTTTG
GGCTTATTATTGGGTTACAAAACCATCTTTGGGCTTATTATTGGGTCACAAAACCATCTT
TGGGCTTATTATCAGTGATCCATAATTATTCCTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTTGATGTCCGCAGCGTTTTTATA
   
Sequence Name 28-K103930w-0022-606-E07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103930w-0022-606-E07C-8409
ATATTATGGATCACTGATAATAAGCCCAAAGATGGTTTTGTGACCCAATAATAAGCCCAA
AGATGGTTTTGTAACCCAATAATAAGCCCAAAGAATGTAAATAGTGAGTTCTTCATGGAC
TAATTCAAGTTTTATTGTATGTGGTGTTGCTGCGTGGGCTACGATGCTTTTCTACATACC
CTTAACAAGATTACGGCAATATTGTGTTATATTTTGTGGATTCTTTCGAAGCTGACATGA
TCTATTGAAACTCTGAATAACCCTGCGCCCGTTCTCGGACATACTTTGGCCATATACACA
ACCTAGTTCGAAGTTTCTAGTGGTGTATAAGTTCAAGGTAGTGATGCCTCAAAGTCAAAG
TAACCGTATAGCTCGTGACATAACGAAGTGTGTGACAAGAGATAGTCGTTTTCAATCTTA
TTGCCCCGTTAAGG

Last Updated on 06.12.2018 16:11