Sequences from confirmation amplicon


Confirmation sequences for line 606E12 and hit At2g13630 are shown.
Line ID 606E12
Locus At2g13630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5685034
Primers used for PCR 66mm - 8474, show primer details
   
Sequence Name 56-K115259-0022-606-E12C-66mm
GenBank Accession LN509194 [GenBank]
Sequencing primer gene specific primer, 66mm: 5'-AAAGGGCAAGGAAGTCTAAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K115259-0022-606-E12C-66mm
GCGTTCAATGAAGTTGCCAACGAAATCCATAGCCTAGCTAGGTTAGGTTTTGAATCACTA
GTCATCGATAATCCGCAGCAAAGACTTCACCAACTTGTACATGACTTGATCTTTGGCTCA
ATTGCGTCTTCTTTTCACAGTCTATCGTCGTAACATGCAGATGCAGCTCTTTCACTCCTG
CTCTCAGGAGGATCCGTCTTTTAATCATCGTACTATGAATTCGGATCTAGGGTACCTGTT
TTCTCCACCCGTCGAGGGTTTGATCTTCTGTAGAAATAATACTAAAGCAATGATTGGAAA
TCCTAGTACGGGTCAGTTTGTATCTTTACTACGAGGAGAAAGAACATATTATCTGTTTTT
GGATATGATCCAGTTAATGATTTGTACAAAGTGTTGTGCAAGACGACAACTAAACGTGGA
TCACCACGTTTTTAATTGGGAAGGTCCTATGTGGGAGGAGTCTATGTCAGAGGAGCATCA
AGTTTTCACTCTAGGAGCAAAACAAAAATGGAGAATGCTCGAATGTAAGTATACTCATCG
TCATTATCCTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 16-K115258-0022-606-E12C-8409
GenBank Accession LN493553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115258-0022-606-E12C-8409
TTGAATATATCCTAGGATAATGACGATGAGTATACTTACATTCGAGCATTCTCCATTTTT
GTTTTGCTCCTAGAGTGAAAACTTGATGCTCCTCTGACATAGACTCCTCCCACATAGGAC
CTTCCCAATTAAAAACGTGGTGATCCACGTTTAGTTGTCGTCTTGCACAACACTTTGTAC
AAATCATTAACTGGATCATATCCAAAAACAGATAATATGTTCTTTCTCCTCGTAGTAAAG
ATACAAACTGACCCGTACTAGGATTTCCAATCATTGCTTTAGTATTATTTCTACAGAAGA
TCAAACCCTCGACGGGTGGAGAAAACAGGTACCCTAGATCCGAATTCATAGTACGATGAT
TAAAAGACGGATCCTCCTGAGAGCAGGAGTGAAAGAGCTGCATCTGCATGTTACGACGAT
AGACTGTGAAAAGAAGACGCAATTGAGCCAAAGATCAAGTCATGTACAAGTTGGTGAAGT
CTTTGCTGCGGATTATCGATGACTAGTGATTCAAAACCTAACCTAGCTAGGCTATGGATT
TCGTTGGCAACTTCATTTGAACGCGGTCGTATTCATCTCTTTTTTCTTCTGGTTTCTTAG
ACTTCCTTGCCCT

Last Updated on 06.12.2018 16:11