Sequences from confirmation amplicon


Confirmation sequences for line 606H01 and hit At4g14080 are shown.
Line ID 606H01
Locus At4g14080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8119176
Primers used for PCR BD53 - 8474, show primer details
   
Sequence Name 22-K028348-022-606-H01C-8409
GenBank Accession LN495976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K028348-022-606-H01C-8409
ATTGAAATATCCTGTGTCCCCGAACCGGATTTCTGGTTTTCGTTGAATAAGGAGAAAACA
AATGTCGGTATTGGTAAACCGGGTCTTGATGGTGTATTGATTGGAGGACTTGCGCTCATC
TTCTTGATCAGATTACGGTTATAGGTAGCTGCGTTGAGAATGTTGGCTCCGGTTTCGTCG
ATGTCACCGAAATTAGGCCATCCGGTTTCAGAGATCGCGAGGCGCATATGTGGATAACCG
AGTTTGGTCATGGCGAAGAGAACCGAATCCAACATTTGGTCTAGAAGATTACGGTAAACC
AAACCGGTTTGAGGATCGGTATAGGTTGAGTGTCCTTGGAACAGAGCAAAATCCAAACTG
GTGTTCATGGGGTTTCTTGACCAACGGAAGTAAGGATGAACATTAAGGAAGAAGTAAGAG
TTTGTT
   
Sequence Name 22-K028348-022-606-H01C-BD53
GenBank Accession LN495977 [GenBank]
Sequencing primer gene specific primer, BD53: 5'-GAAGTTTCTCAACGGAACAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K028348-022-606-H01C-BD53
GACACTCAACCTATACCGATCCTCAAACCGGTTCGGTTTACCGTAATCTTCTAGACCAAA
TGTTGGATTCGGTTCTCTTCGCCATGACCAAACTCGGTTATCCACATATGCGCCTCGCGA
TCTCTGAAACCGGATGGCCTAATTTCGGTGACATCGACGAAACCGGAGCCAACATTCTCA
ACGCAGCTACCTATAACCGTAATCTGATCAAGAAGATGAGCGCAAGTCCTCCAATCAATA
CACCATCAAGACCCGGTTTACCAATACCGACATTTGTTTTCTCCTTATTCAACGAAAACC
AGAAATCCGGTTCGGGGACACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11