Sequences from confirmation amplicon


Confirmation sequences for line 607A07 and hit At4g31250 are shown.
Line ID 607A07
Locus At4g31250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15180609
Primers used for PCR EU19 - 8474, show primer details
   
Sequence Name 03-K103353-0022-607-A07C-EU19
GenBank Accession LN487449 [GenBank]
Sequencing primer gene specific primer, EU19: 5'-CTGAAGGTTCATCTTGAAGGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103353-0022-607-A07C-EU19
GGGAATGCCGAAGCTTACTGAGCTGAATCTTGAGGATAATATGTTCACCGGGAAAATACC
GGCGTTTAAACAGAAGAATCTCGTCACCGTTAACGTTGCTAACAATCAGTTAGAGGGTCG
AATTCCGTTAACTCTCGGCCTTATGAACATCACTTTCTTCTCAGGTATGAATCATCATCA
TCATCAAATCTGTTTATTTTTCTTTGATTAATTATCATCTGAACATCTTTCATATTATAA
TCTGATGTATATATATATATATATATATATATATATATATATATACTGGCAACACACAAT
ATTTGTGAAAATTTTCCTAAGGACAAGACTTTATGTATTTATCTCTATTCGTGGGAAAAT
TTAAACATATTGGTCTTGGACCCTTTGGTGAGTAGGAGTGAGCACGTTCTCACGCATTAA
ATTGTGCGTGAAAAAAAAAAAAATGTGTGCGTGTAAAAGATACAATATGAAATTTTTTTT
CTTCTCTTGACAAAATATGTGTGGAAAACACTCTCTTAGAAAAAATTATGAGGGGGCCTA
TAGATAAGTGGGCCGTGTATGATGTTAAATAAATAATTCGTGTGTAAATGGCTTCATGCC
CGGGAAATCTATATGGATCAGCAGTGAGTGTGAGGGTCTATATAGAGAAAAAAAAAGAGT
AATCCCCTTTTTTTTTTCAATTCAAAAATGTATATGTCCGCAGCTTATATAT
   
Sequence Name 19-K103354-0022-607-A07C-8409
GenBank Accession LN494722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103354-0022-607-A07C-8409
AAACGAATTATTTAATTAACATCATACATGGCCCATTTATCAATAGGCCCACTAATAATC
ATTTCAAAGAGAGTATTTTCCACAAATATTTTGTCAAAAGAAGAAATAAAATTTCATATT
GTAACTTTTACATGCAAACATTTTTTTTTATTACATGCAAAATTTAATGCCTAATAACGT
GCTCACTACTACTCACCAAAGGCTCCAAGACCAATATGTTCAAATTTTCCCATGAATAGT
GATAATTACATAAAGTCTAGTCCTTAGGAAAATTTTCACAAATGTTGTGTGTTGCCAGTA
TATATATATATATATATATATATATATATATATATACATCAAATTATAATAAGAAATATG
TTCATATGATAATAACTCAAAAAAAAAAAAACAGATATTATGAAGATGATCATACACACC
TGAAAAAAAAGATATGTACATAAGGGCGAGAGTTAACAGAATTCCCCCCTCTAACTGATT
GATCGCAACGTTAACGGAGACGAGATTCTTCTGTATAAACGCCTGTTTTTTCCCGGAGAA
CATATTATCCTCAAGATACAGCTCAGAAAGCTTCGGCAATTTCTCTAAAGACTCCAGAAT
CTCACCAGAAAACCTGTTTCTTTCAAGATAAACCTACAGAGA
Primers used for PCR 68wn - 3144, show primer details
   
Sequence Name 29-K118466-0022-607-A07C-68wn
GenBank Accession LN499485 [GenBank]
Sequencing primer gene specific primer, 68wn: 5'-CAACAAAACTCATCAGATTCCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118466-0022-607-A07C-68wn
TTAGAGAGTTCCATAATATTACCATGGAGGAGATTAGCGAGGCTTCCATTGGAAATGTAA
TTGGTAACGAGAAGCTTCTCTTCTTTTCTGTAATAGAATGCAATCAACGGAAGGAGATTA
GGGTGAGACAATCGTCCGATCTTCTTCATATGATCGTAAAACTCTTCTCTCCCGATATTA
CTCATAAACCTAAACCGTTTCACAACCACCGCACGACCACTCGATAAAGCCGCTTTGTAC
GAAGATCCAAATCCGCCGCTTCCGAGAACCTCCGCCGATGCACGGAGCATATCCTGAAGC
GTGAACCTCTCCTGATCATTCCTAACGAAATGTAGCTTCCGTTGATCTCCTCGTTTGTCT
TCATCTGGAGAGAGACCTCCCACGGATATAGCTCCTGATGTTGCTGTTGAATCTCGCTGC
ACAGTTTCATTAGCTAGTTTTCTGTAGACCTTGGAGTCCTGTGAGCTCTTTTCACTGTGT
TGTTGTTGCTCGGGTTGTCCGTAGACTTGTCCGTGAAAATGGCCGACGCCGTGGTTTTGG
ATTTGGTCTTGTCCTTTGCCTTGACGGCGGCTGAGAATGCAAACGGAAAGAAAGACGGTA
ATGAGCACTACGACGGCGAGGATGGTGAGGGCAAGGAGAAACACGGTGAAGAACGGCGGA
CGAGTGTATCAATGGCGACTACGCGCAGCAATGAAACACTGATAGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTG
   
Sequence Name 02-K118465-0022-607-A07C-35St
GenBank Accession LN487237 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118465-0022-607-A07C-35St
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCAT
TGCTGCGCGTAGTCGCCATTGATACACTCGTCCGCCGTTCTTCACCGTGTTTCTCCTTGC
CCTCACCATCCTCGCCGTCGTAGTGCTCATTACCGTCTTTCTTTCCGTTTGCATTCTCAG
CCGCCGTCAAGGCAAAGGACAAGACCAAATCCAAAACCACGGCGTCGGCCATTTTCACGG
ACAAGTCTACGGACAACCCGAGCAACAACAACACAGTGAAAAGAGCTCACAGGACTCCAA
GGTCTACAGAAAACTAGCTAATGAAACTGTGCAGCGAGATTCAACAGCAACATCAGGAGC
TATATCCGTGGGAGGTCTCTCTCCAGATGAAGACAAACGAGGAGATCAACGGAAGCTACA
TTTCGTTAGGAATGATCAGGAGAGGTTCACGCTTCAGGATATGCTCCGTGCATCGGCGGA
GGTTCTCGGAAGCGGCGGATTTGGATCTTCGTACAAAGCGGCTTTATCGAGTGGTCGTGC
GGTGGTTGTGAAACGGTTTAGGTTTATGAGTAATATCGGGAGAGAAGAGTTTTACGATCA
TATGAAGAAGATCGGACGATTGTCTCACCCTAATCTCCTTCCGTTGATTGCATTCTATTA
CAGAAAAGAAGAGAAGCTTCTCGTTACCAATTACATTTCCAATGGAAGCCTCGCTAATCT
CCTCCATGGTAATATTATGGAACTCTCTAAGTGTCTGTTTATCTTCATCCAATGAAATGA
GGATCTAGAATTTTTT

Last Updated on 06.12.2018 16:11