Sequences from confirmation amplicon


Confirmation sequences for line 607B12 and hit At1g72040 are shown.
Line ID 607B12
Locus At1g72040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27112891
Primers used for PCR LD19 - 8474, show primer details
   
Sequence Name 73-K030473-022-607-B12C-8409
GenBank Accession LN512819 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K030473-022-607-B12C-8409
AGACAACCGGTATCAAACCAAGAGTCGCAATAGCTGATCTCCATCTCATTCATCCATTTC
GCTTCATGAACCGCACGCACAAACACCTAAACACCAAATTCCACAACATATTAAGTTTCC
AATCACACATTTCAAACTATAAATTTTAATAACGTTGGCTTTAAAATTACCATTCGGTCA
CTGAAGACACTCCTTTCCATCAACCTGAGAGGTTTAACCCCAGAAGCAGACTCTTTCTCC
TGCATCAGCCGAGTGACAAACACATAGTTCTGGAAAGTATAAGCATACCTCTGAGGCTCA
GAGTAGAAAGCATCCAATATATTGAAATGGTCAGGTCCAACATCTTGCCACTTATCAACT
GGCTCAGGAACAATCTCAACAAGGTCTTGTAACTCAACAGTCTCATTCGCTATCCTCTGA
AGAAAAGTTGATTTACCAA
   
Sequence Name 74-K030473-022-607-B12C-LD19
GenBank Accession LN512991 [GenBank]
Sequencing primer gene specific primer, LD19: 5'-AGGGAACATCAGTGTTGGTAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K030473-022-607-B12C-LD19
GACCATTTTCAATATATCGGAACGCTTTCTACTCTGAGCCTCAGAGGTACGCTTATACTT
TCCAGAAACTATGTGTTTGTCACTCGGCTGATGCAGGAGAAAGAGTCTGCTTCTGGGGTT
AAACCTCTCAGGTTGATGGAAAGGAGTGTCTTCAGTGACCGAATGGTAATTTTAAAGCCA
ACGTTATTAAAATTTATAGTTTGAAATGTGTGATTGGAAACTTAATATGTTGTGGAATTT
GGTGTTTAGGTGTTTGTGCGTGCGGTTCATGAAGCGAAATGGATGAATGAGATGGAGATC
AGCATTTACGACTCTTGGTTTGATCCGGTTGTCTCTTCTTTACCTGGACTACAATTGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11