Sequences from confirmation amplicon


Confirmation sequences for line 607D07 and hit At2g24330 are shown.
Line ID 607D07
Locus At2g24330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10348898
Primers used for PCR CH69 - 8474, show primer details
   
Sequence Name 28-K105918-0022-607-D07C-CH69
GenBank Accession LN498849 [GenBank]
Sequencing primer gene specific primer, CH69: 5'-TTCATAACCACCCTTTATCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105918-0022-607-D07C-CH69
TGAGCACTAGTTTCACAGTTACAAGCTAGCGCCTAGCGGAACTTGAAAAGCGAAAAAGAG
AGACTCTCAATAAGGATAATCCTTGTATAATGGCTGTACTGTCCTAAAGGAATTCCAATA
CAAATTTTTGAATACCTAGCTCTTTGCTAAGGTTAAATTTAAGGTCATCTTCTAATTCAA
CAAAGACGGATAACTTGCAACATAAAACAGAAATGAAAAATGATCTAATCCTACGACAAA
GCTAGAATCTTTTAAGTGATCTGTATCTCTATCAGAACTAGGTAAAGTAGTGCAAGCAAA
GAATAACAGCAGCACACATCAAAACATGACAATTAAAATAATCAGGAATACCAGAAACAC
CAATAGAATTCGAATGAACTAGCCTCACTCACACAGAACAAGTTGTATCTTACCTGAATA
AGTTGCTGTGTAATGTAATAATTGGTCCTTTCCTTTCCTTTAACTCATTACCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCC
   
Sequence Name 28-K105919-0022-607-D07C-8409
GenBank Accession LN498850 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105919-0022-607-D07C-8409
GAGCCATTTACAATTGAATATATCCTGGTAATGAGTTAAAGGAAAGGAAAGGACCAATTA
TTACATTACACAGCAACTTATTCAGGTAAGATACAACTTGTTCTGTGTGAGTGAGGCTAG
TTCATTCGAATTCTATTGGTGTTTCTGGTATTCCTGATTATTTTAATTGTCATGTTTTGA
TGTGTGCTGCTGTTATTCTTTGCTTGCACTACTTTACCTAGTTCTGATAGAGATACAGAT
CACTTAAAAGATTCTAGCTTTGTCGTAGGATTAGATCATTTTTCATTTCTGTTTTATGTT
GCAAGTTATCCGTCTTTGTTGAATTAGAAGATGACCTTAAATTTAACCTTAGCAAAGAGC
TAGGTATTCAAAAATTTGTATTGGAATTCCTTTAGGACAGTACAGCCATTATACAAGGAT
TATCCTTATTGAGAGTCTCTCTTTTTCGCTTTTCAAGTTCCGCTAGGCGCTAGCTTTGTA
ACTGTGAAACTAGTGGCTCAATGTGTTAGGCAAAGCTATATTGTAAGTTTGGATAAAGGT
GGGTTTATGAA

Last Updated on 06.12.2018 16:11