Sequences from confirmation amplicon


Confirmation sequences for line 608E12 and hit At5g62980 are shown.
Line ID 608E12
Locus At5g62980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25276885
Primers used for PCR CH70 - 8474, show primer details
   
Sequence Name 29-K105918-0022-608-E12C-CH70
GenBank Accession LN499291 [GenBank]
Sequencing primer gene specific primer, CH70: 5'-GTGTCTCAAAAAGGCTGGTCTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105918-0022-608-E12C-CH70
GTCGACATTTACAGTTAGTTTAATTACTAATATGAGAGGATTTGCTAGAGATAGTTAACT
AAATTCTCCCCTTTACTCTTGACCAATCCATTTTTATTGTGACCTCATCCAAAAATGACA
AGCTTTGCTTATATAACAATTTGTCATCACTATCTGTGTCACTGAGTGATGCATTGATTA
TAGGATATGAAATGATTCTTTGAGATTGAAGATTTGAAAAGGTTGTGTGTAGGTTATGTA
GTAGTGACTACACTTTTCATATGCTGTGTTTGAAACTGTATCATAATTTGTTTTGGAATG
GAATGAATAATCTTAGCTTATATTCAATTGAATATTTACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 29-K105919-0022-608-E12C-8409
GenBank Accession LN499292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105919-0022-608-E12C-8409
TCGGACATGAGCCATTTACATTGAATATATCCTGTAAATATTCAATTGAATATAAGCTAA
GATTATTCATTCCATTCCAAAACAAATTATGATACAGTTTCAAACACAGCATATGAAAAG
TGTAGTCACTACTACATAACCTACACACAACCTTTTCAAATCTTCAATCTCAAAGAATCA
TTTCATATCCTATAATCAATGCATCACTCAGTGACACAGATAGTGATGACAAATTGTTAT
ATAAGCAAAGCTTGTCATTTTTGGATGAGGTCACAATAAAAATGGATTGGTCAAGAGTAA
AGGGGAGAATTTAGTTAACTATCTCTAGCAAATCCTCTCATATTAGTAATTAAAACTAAC
TTGTAAATGTCGACATAGCTGACAGAATCAGCTAAGTTGTCTGATAGACCAGCCTTTTTG
AGACA

Last Updated on 06.12.2018 16:11