Sequences from confirmation amplicon


Confirmation sequences for line 608F01 and hit At1g67090 are shown.
Line ID 608F01
Locus At1g67090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25049115
Primers used for PCR ND03 - 8474, show primer details
   
Sequence Name 21-K032005-022-608-F01C-8409
GenBank Accession LN495556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K032005-022-608-F01C-8409
CTTCATCCAAGCACGGCGGAAGAGTTAACTGCATGCAGGTCATTTATATTTCTTCTTTCA
CTTTTTTATTATTCCATATGATTTTTTTCGGTTCTTTCTTCGAATCTACATAAACTAATA
TCATTGGAAAAATCGAAAAAATAGGTGTGGCCTCCGATTGGAAAGAAGAAGTTTGAGACT
CTCTCTTACCTTCCTGACCTTACCGATTCCGAATTGGCTAAGGAAGTTGACTACCTTATC
CGCAACAAGTGGATTCCTTGTGTTGAATTCGAGTTGGAGGTAATTAAACAAAATTTAAAC
ATCTATATAAACTAGCTAGATCTTAGGAAAATTTGGTTTAATATATTAGGATCTTGATTT
ATATAAACATGTTCAAAATGTTATCTGAGTGGTTTGTAACATGTGGTT
   
Sequence Name 22-K032005-022-608-F01C-ND03
GenBank Accession LN496002 [GenBank]
Sequencing primer gene specific primer, ND03: 5'-AAACCACATGTTACAAACCACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K032005-022-608-F01C-ND03
AATCAGATCCTAATATATTAAACCAAATTTTCCTAAGATCTAGCTAGTTTATATAGATGT
TTAAATTTTGTTTAATTACCTCCAACTCGAATTCAACACAAGGAATCCACTTGTTGCGGA
TAAGGTAGTCAACTTCCTTAGCCAATTCGGAATCGGTAAGGTCAGGAAGGTAAGAGAGAG
TCTCAAACTTCTTCTTTCCAATCGGAGGCCACACCTATTTTTTCGATTTTTCCAATGATA
TTAGTTTATGTAGATTCGAAGAAAGAACCGAAAAAAATCATATGGAATAATAAAAAAGTG
AAAGAAGAAATATAAATGACCTGCATGCAGTTAACTCTTCCGCCGTTGCTTGTGATGGAA
GTAATGTCGTTGTTAGCAATGAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGT
Primers used for PCR FP94 - 3144, show primer details
   
Sequence Name 17-K033041-022-608-F01C-FP94
GenBank Accession LN493752 [GenBank]
Sequencing primer gene specific primer, FP94: 5'-TAAGGGTCTCAACACCTCTCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K033041-022-608-F01C-FP94
AAATTAACCTCACACATATCCACACTCAAAATCCAACGGTGTAGATCCTAGTCCACTTGA
ATCTCATGTATCCTAGACCCTCCGATCACTCCAAAGCTTGTTCTCATTGTTGTTATCATT
ATATATAGATGACCAAAGCACTAGACCAAACCTCAGTCACACAAAGAGTAAAGAAGAACA
ATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCGGCTCAGGCCACTATG
GTCGCTCCTTTCAACGGACTTAAGTCCTCCGCTGATAGTTTAAACCGAAGGCGGGAAACG
ACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGG
AAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
ATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCG
TGGGT
   
Sequence Name 49-K033041-022-608-F01C-3144
GenBank Accession LN506742 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K033041-022-608-F01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGCGGAGGAC
TTAAGTCCGTTGAAAGGAGCGACCATAGTGGCCTGAGCCGGAGAGGCAACCATAGTAGCG
GAAGAGAGCATAGAGGAAGCCATTGTTCTTCTTTACTCTTTGTGTGACTGAGGTTTGGTC
TAGTGCTTTGGTCATCTATATATAATGATAACAACAATGAGAACAAGCTTTGGAGTGATC
GGAGGGTCTAGGATACATGAGATTCAAGTGGACTAGGATCTACACCGTTGGATTTTGAGT
GTGGATATGTGTGAGGTTAATTTTACTTGGTAACGGCCACAAAGGCCTAAGGAGAGGTGT
TGGNNACCCTT

Last Updated on 06.12.2018 16:11