Sequences from confirmation amplicon


Confirmation sequences for line 608F07 and hit At5g03040 are shown.
Line ID 608F07
Locus At5g03040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:711266
Primers used for PCR 16gm - 8474, show primer details
   
Sequence Name 18-K111730-0022-608-F07C-16gm
GenBank Accession LN494365 [GenBank]
Sequencing primer gene specific primer, 16gm: 5'-CTACCAGCCATCCATCTCTCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K111730-0022-608-F07C-16gm
CATCGGGTTTCCGATTTAGAGTTGTTCTTCCAGTTTTGCTGCAAACATGAACATATAGGA
TCATTACAACATTCAAATGAACCACAGATTCAACTTCAATGGATGTGTTCACTTTTTAAT
TGTATCTTTCCACACATTCAGCATTATAGGTAACGACTGGAGTACCTGATGAGAGTATGA
ATAAGCCAATGCCCTTTCCCTTCTCATTGTTGCCTCGTACTTGCTTAGCAAATTCGCTTC
AACTTTCTCCTTTGATTGAATGCTATCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K111729-0022-608-F07C-8409
GenBank Accession LN487095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K111729-0022-608-F07C-8409
ATTTACATTGATAGCATTCAATCAAAGGAGAAAGTTGAAGCGAATTTGCTAAGCAAGTAC
GAGGCAACAATGAGAAGGGAAAGGGCATTGGCTTATTCATACTCTCATCAGGTACTCCAG
TCGTTACCTATAATGCTGAATGTGTGGAAAGATACAATTAAAAAGTGAACACATCCATTG
AAGTTGAATCTGTGGTTCATTTGAATGTTGTAATGATCCTATATGTTCATGTTTGCAGCA
AAACTGGAAGAACAACTCTNAATCTGGAAACCCGATGTTCATGGATCCAAGCAACCCGAC
ATGGGGTTGGAGCTGGTTAGAGAGATGGAGGGCTGGTAG

Last Updated on 06.12.2018 16:11