Sequences from confirmation amplicon


Confirmation sequences for line 609H08 and hit At5g24000 are shown.
Line ID 609H08
Locus At5g24000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8110931
Primers used for PCR KA24 - 8474, show primer details
   
Sequence Name 41-K105566-0022-609-H08C-KA24
GenBank Accession LN504080 [GenBank]
Sequencing primer gene specific primer, KA24: 5'-AGCTTCAGAATCCTCCAATCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K105566-0022-609-H08C-KA24
ATCGTTTGAGAAAAACTCCACATCCAATGCCTAGAAAAGTTGCTATGGAGACCCCAAAAA
CACCAGAGAGAATCCCGGGGACGACAAGAGAAGTCCATCCTTTTGCGGTTGCCATGGCAC
ACGCAGTGGTTGGGCCTCCGATGTTAGCATTTGATGCAAGAAGTAATAGCTTCATGTCGA
TACAGAATAGTTTTCCTAGAACCAAAGTCACTGCTAGATGAACCATTACTTGAATAGCAG
CAAATAAGAAGATACTTGGGGCAGTGTTGATTACATTCCATACACTCCCTGTGGCTCCTA
GAATTGTGAAAAATACCTGAACAAAAAAGATTCAAAAACAAACCATGGTCTGAAGTCCTC
GGGCTTAATCAGGGATGGGCTTTAACAATGAGAATAAGGGCTCAAAACGGGTCAGACATA
GCCCATTTTGCCAATGACATGTCTAATTGGTTCTAAATAATGGATAAAGTGTAAAGAAAG
AGATACCTGCATGAGAATGAGAGAGATGGTTTCAGCAGAAGGTGCCAGAGAGTTGAAGAC
ATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTC
   
Sequence Name 73-K105567w-0022-609-H08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K105567w-0022-609-H08C-8409
GCCTTTACAATTGAATATGATGTCTTCACTCTCTGGCACCTTCTGCTGAAACCATCTCTC
TCATTCTCATGCAGGTATCTCTTTCTTTACACTTTATCCATTATTTAGAACCAATTAGAC
ATGTCATTGGCAAAATGGGCTATGTCTGACCCGTTTTGAGCCCTTATTCTCATTGTTAAA
GCCCATCCCTGATTAAGCCCGAGGACTTCAGACCATGGTTTGTTTTTGAATCTTTTTTGT
TCAGGTATTTTTCACAATTCTAGGAGCCACAGGGAGTGTATGGAATGTAATCAACACTGC
CCCAAGTATCTTCTTATTTGCTGCTATTCAAGTAATGGTTCATCTAGCAGTGACTTTGGT
TCTAGGAAAACTATTCTGTATCGACATGAAGCTATTACTTCTTGCATCAAATGCTAACAT
CGGAGGCCCAACCACTGCGTGTGCCATGGCAACCGCAAAAGGATGGACTTCTCTTGTCGT
CCCCGGGATTCTCTCTGGTGTTTTTGGGGTCTCCATAGCAACTTTTCTAGGCATTGGATG
TGGAGTTTTTGTCCTCAAACGATTATAGATTTCTAAACAGTTCTAATGATTG
Primers used for PCR AP27 - 3144, show primer details
   
Sequence Name 02-K105974-0022-609-H08C-AP27
GenBank Accession LN487039 [GenBank]
Sequencing primer gene specific primer, AP27: 5'-GCATTTGCATCCACATATAAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105974-0022-609-H08C-AP27
TGCTGACATGACTCAGGATGACAAGCTCGAGGACAAGAACAGAGCCGTTTCAACTTCAAT
TGCACTATCAGTTTCCTTCCTGATATGCAAAGCTGCAATTACGTTGACGACGCTATTTAA
GATACAAGGAGTTATGCTTCCTGCAGTAACAGCCATCACCATTGTCTTGGCAACATCCTT
CCAAAATGGCTTCATGTCCGGGAAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAA
ATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCC
TTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 12-K105975-0022-609-H08C-3144
GenBank Accession LN491582 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105975-0022-609-H08C-3144
AGATTTCCCGGACATGAAGCCATTTTGGAAGGATGTTGCCCAGACTAAGGTGATGGCTGT
TACTGCAGGAAGCATAACTCCTTGCATCTTAAATAGCGTCGTCAACGTAATTGCTGCTTT
GCTTATCATGGAAGGAAACTGATAGAGCAATTGAAGTTGAAACCACTCTCTTCTTGTCCT
CGAGCTTGTCATCCTTAGTTACGTCAGCATCTGCCAAAGATCACAAAGACTTGAATAATT
CTGTTTATATGTGGATGCAAATGCAT

Last Updated on 06.12.2018 16:11