Sequences from confirmation amplicon


Confirmation sequences for line 610E07 and hit At1g62610 are shown.
Line ID 610E07
Locus At1g62610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23181714
Primers used for PCR 02rk - 8474, show primer details
   
Sequence Name 33-K118107-0022-610-E07C-02rk
GenBank Accession LN501176 [GenBank]
Sequencing primer gene specific primer, 02rk: 5'-AGAGGCAATGTCAAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118107-0022-610-E07C-02rk
GTCTTTAGACCAACTTGACCGGATCTTGGTTAATATCCAATACGTCTGCCTTTTGATGCG
TGACGCTAAGCGTGGGGGCTCGGTGATTAACGTCTCGTCGATCTCTGGGCTTCATCGCGG
TTTGTTGCGCGGTGGACTAGCGTATGCTTGTTCCAAAGGTGGTGTTGACACCATGACGAG
GATGATGGCTATTGAGTTAGCTGTTTATAAGATCAGAGTGAACTCGATCGCACCTGGGAT
TTTCAGGTCAGAGATCACACAAGGTCTTTTCCAGAAATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTTATT
   
Sequence Name 14-K118106-0022-610-E07C-8409
GenBank Accession LN492706 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118106-0022-610-E07C-8409
CATTGAATATTTCTGGAAAGACCTTGTGTGATCTCTGACCTGAAAATCCCAGGTGCGATC
GAGTTCACTCTGATCTTATAAACAGCTAACTCAATAGCCATCATCCTCGTCATGGTGTCA
ACACCACCTTTGGAACAAGCATACGCTAGTCCACCGCGCAACAAACCGCGATGAAGCCCA
GAGATCGACGAGACGTTAATCACCGAGCCCCCACGCTTAGCGTCACGCATCAAAAGGCAG
ACGTATTTGGATATTAACCAAGATCCGGTCAAGTTGGTTCTAAAGACTTTGTCCCATTCT
TCTTTTGATAAATCCAAACTAGACTTGAAATTGCCTCT

Last Updated on 06.12.2018 16:11