Sequences from confirmation amplicon


Confirmation sequences for line 611F01 and hit At3g59360 are shown.
Line ID 611F01
Locus At3g59360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21939589
Primers used for PCR MP50 - 8474, show primer details
   
Sequence Name 11-K025266-022-611-F01C-8409
GenBank Accession LN490946 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K025266-022-611-F01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGGTAGCAAAACTATTTCTAGGGCTTAT
GACGACCACAAGATTAGGGTATCCTCAAAACAACGTGTCCTCAATGTCCTTTTGGTTGTA
GGTGATTGCATGCTTGTTGGTCTCCAGGTAAATCATCTCCGTTTGACAAATTTTCCATAT
TTATTATCAGCGTTCTTCTTTTTCCTCTCAGCTCATCATTTGTTTGTGTAAATCACTTAA
AAGTTACATACTTTTCCTCTTTTTCAGCCTGTCTTGGTGTATATGTCGAAGGTGGATGGG
AAATTCAACTTCAGTCCTATTAGCGTCAACTTCTTGACAGAAATCGCAAAAGTTATTTTT
GCGATTGTGATGCTTT
   
Sequence Name 12-K025266-022-611-F01C-MP50
GenBank Accession LN491405 [GenBank]
Sequencing primer gene specific primer, MP50: 5'-ACCTGGATTAAAAGCATCACAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K025266-022-611-F01C-MP50
TAACTTTTGCGATTTCTGTCAAGAAGTTGACGCTAATAGGACTGAAGTTGAATTTCCCAT
CCACCTTCGACATATACACCAAGACAGGCTGAAAAAGAGGAAAAGTATGTAACTTTTAAG
TGATTTACACAAACAAATGATGAGCTGAGAGGAAAAAGAAGAACGCTGATAATAAATATG
GAAAATTTGTCAAACGGAGATGATTTACCTGGAGACCAACAAGCATGCAATCACCTACAA
CCAAAAGGACATTGAGGACACGTTGTTTTGAGGATACCCTAATCTTGTGGTCGTCATAAG
CCCTAGAAATAGTTTTGCTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACTTTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11