Sequences from confirmation amplicon


Confirmation sequences for line 611F04 and hit At1g07240 are shown.
Line ID 611F04
Locus At1g07240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2225302
Primers used for PCR 12vo - 8474, show primer details
   
Sequence Name 57-K118077-0022-611-F04C-12vo
GenBank Accession LN509539 [GenBank]
Sequencing primer gene specific primer, 12vo: 5'-GGCCCAATATAAAGAGATGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118077-0022-611-F04C-12vo
CGGGAGCATGGGAGTCTTCCCTGCACCTCAGATCACAGAGATTGCTCACGCGCTCGAGCT
TATCGGGTGCAGGTTCATCTGGGCGATCCGTACGAACATGGCGGGAGATGGCGATCCTCA
GGAGCCGCTTCCAGAAGGATTTGTCGATCGAACAATGGGCCGTGGAATTGTGTGTAGTTG
GGCTCCACAAGTGGATATCTTGGCCCACAAGGCAACAGGTGGATTCGTTTCTCACTGCGG
GTGGAATTCCGTCCAAGAGAGTCTATGGTACGGTGTACCTATTGCAACGTGGCCAATGTA
TGCGGAGCAACAACTGAACGCATTTGAGATGGTGAAGGAGTTGGGCTTAGCAGTGGAGAT
AAGGCTTGACTACGTGGCGGATGGTGATAGGGTTACTTTGGAGATCGTGTCAGCCGATGA
AATAGCCACAGCCGTCCGATCATTGATGGATAGTGATAACCCCGTGAGAAAGAAGGTTAT
AGAAAAATCTTCAGTGGCGAGGAAAGCTGTTGGTGATGGTGGGTCTTCTACGGTGGCCAC
ATGTAATTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATT
   
Sequence Name 32-K118076-0022-611-F04C-8409
GenBank Accession LN500753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118076-0022-611-F04C-8409
ACATTGAATATATCCTGTAAAATTACATGTGGCCACCGTAGAAGACCCACCATCACCAAC
AGCTTTCCTCGCCACTGAAGATTTTTCTATAACCTTCTTTCTCACGGGGTTATCACTATC
CATCAATGATCGGACGGCTGTGGCTATTTCATCGGCTGACACGATCTCCAAAGTAACCCT
ATCACCATCCGCCACGTAGTCAAGCCTTATCTCCACTGCTAAGCCCAACTCCTTCACCAT
CTCAAATGCGTTCAGTTGTTGCTCCGCATACATTGGCCACGTTGCAATAGGTACACCGTA
CCATAGACTCTCTTGGACGGAATTCCACCCGCAGTGAGAAACGAATCCACCTGTTGCCTT
GTGGGCCAAGATATCCACTTGTGGAGCCCAACTACACACAATTCCACGGCCCATTGTTCG
ATCGACAAATCCTTCTGGAAGCGGCTCCTGAGGATCGCCATCTCCCGCCATGTTCGTACG
GATCGCCCAGATGAACCTGCACCCGATAAGCTCGAGCGCGTGAGCAATCTCTGTGATCTG
AGGTGCAGGGAAGACTCCCATGCTCCCGAAACACAGGAACAAAACCGACGAGTCTGGTTG
CTCGTCAAGCCACTTCATCATCTCTT

Last Updated on 06.12.2018 16:11