Sequences from confirmation amplicon


Confirmation sequences for line 612C01 and hit At3g62740 are shown.
Line ID 612C01
Locus At3g62740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23211636
Primers used for PCR 30qr - 8474, show primer details
   
Sequence Name 60-K115714-0022-612-C01C-30qr
GenBank Accession LN510339 [GenBank]
Sequencing primer gene specific primer, 30qr: 5'-TTTTTACGAGTGGAAAATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K115714-0022-612-C01C-30qr
GACTAAACACTAAACATGTCTTGTAATTAATCAAAGACGAAAGGAGGAAACAAAAATGAA
ACCTTTCTCTCAATTTTTCGTATTTGTGGTTACCGTTTCCGCGACAAGCTACATTGATGC
CTTCACTAGAAACGATTTTCCAAATGATTTCCTCTTCGGAGCCGCCACTTCTGCTTACCA
AGTTCGACTCTGTTTCTGTGTTTTGTATTGATTATAAAATTAAGTTTTTTTTTTGGTTCT
CTGAATGATGTTGGTGGAAATAGTGGGAAGGAGCTTTTGATGAAAACGGAAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCATTATA
   
Sequence Name 20-K115713-0022-612-C01C-8409
GenBank Accession LN495352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115713-0022-612-C01C-8409
AAGCTCCTTCCACTATTTCCACCAACATCATTCAGAGAACCAAAAAAAAAACTTAATTTT
ATAATCAATACAAAACACAGAAACAGAGTCGAACTTGGTAAGCAGAAGTGGCGGCTCCGA
AGAGGAAATCATTTGGAAAATCGTTTCTAGTGAAGGCATCAATGTAGCTTGTCGCGGAAA
CGGTAACCACAAATACGAAAAATTGAGAGAAAGGTTTCATTTTTGTTTCCTCCTTTCGTC
TTTGATTAATTACAAGACATGTTTAGTGTTTAGTCGTGTTCTTGGACTGTTTAAGTAGTG
AGTGATAGGTGGATCTTTTCTATTCCATTTTCCACTCGTAAAAAA

Last Updated on 06.12.2018 16:11