Sequences from confirmation amplicon


Confirmation sequences for line 612E12 and hit At5g10110 are shown.
Line ID 612E12
Locus At5g10110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3166880
Primers used for PCR 26sf - 8474, show primer details
   
Sequence Name 34-K116518-0022-612-E12C-26sf
GenBank Accession LN501561 [GenBank]
Sequencing primer gene specific primer, 26sf: 5'-TTGAAATGAGTAGATGCTCCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K116518-0022-612-E12C-26sf
TGACTTGCTTAATCATGCTGTTGTTCATTCTGTCTCACAGGTGTTTGATTTTTCAGCTTT
TTCTGACTTATCGTTTCTCTTGATTTCCATGAACATTATCTAACTTAGCTACTGCTATCA
ATGAATTGCTTCAGCTTCGTATATTTGGTCAGATCTTAATTACCAGGATAGCAAAGGACG
ATTATCCGGTAAGTCCCGATTATCTTCATATGTATAAAGCATATATTTGCCACAAGCTCT
TGAAGATTTTCTTGTTATTATTGTCATAAGATCTGTTTTTTTTTTTGTTCCTCCGAACCC
ACCCTTGAAAAAAATTCAAAAAGCACCTAGCACCTTGATTCAGGGGAATGTTAAAAAAAA
ATCTACAAATTTTCAGGCCACGGGAAACTCATTTTCTGCAAAACAAAAATCCAAGCTCAG
GGCCCAGGGAAGGTCCCTTTTTCTTACCAGGGGCAATATCCTCAGGATATTTTCAATTGA
AAAGGGCTTCATGTCCGGGAAATCTCCTGGGATCACCATTGAGTATAAGGGTCAATATGG
AAAAAAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCAAA
   
Sequence Name 08-K116517-0022-612-E12C-8409
GenBank Accession LN489892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116517-0022-612-E12C-8409
GCATTTACATTGAAATATCCTGAGGATATCGCCCATGCTAAGAAAGAGCGACCTCCACTG
GGCCATGAGCTTGGATTCTTGTTTTGCAGAAACTGAGTTTCCCGTGGCATGAAAATCTGT
AGATCTTTCTTTAACATTCCCTTGAATCAAGTTGCTAGCTGCTTTCTGACTCTTCTTCAT
GGCTGGTTCAGAAGAAACAAAAAAAAAAACAAATTTTTTGACAATAATACCAAGAAATTC
TTCAAAAGCTTGGGGCAAATATTTGCTTTATACATATAAAAATAATCGGGACTTACCGAA
AAATCGCCCTTTGCTATCCGGGTAATTAAAATCTGACCAAATATCCAAACCTAAACCATT
TCATTGATAGCATTACCTAATTTAAATAATGTTCAGGGAATTCAAAAAAAACAATAATTC
AAAAAAAGCTAAAAAATCAAACCCCTGTGAAACAAAAGGAACAACACCTTGATCTAACCA
AGTCATCTCCGGTATTCAAATTCACATGCTCCTTATTCTAAACATTGAACCACCTCCCCA
TTTCAAA

Last Updated on 06.12.2018 16:11