Sequences from confirmation amplicon


Confirmation sequences for line 614C01 and hit At2g25940 are shown.
Line ID 614C01
Locus At2g25940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11064592
Primers used for PCR 06ok - 8474, show primer details
   
Sequence Name 43-K114805-0022-614-C01C-06ok
GenBank Accession LN504938 [GenBank]
Sequencing primer gene specific primer, 06ok: 5'-TCGGTGAGTGATCTCCTCTATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114805-0022-614-C01C-06ok
ATTTGAATCTCCATGTTTATTTTCAGGGGATGCCAACTTCTCCAAACCTATATGCAAATG
ATCTCAATGATGTCTTGAAGAAAAAATATGCTTCAGGAACATATAAGAGCTTGGTACAAT
GCTCTTTTGAAAATGCTTTCTTTGTCAGCTACATGTGATTTTGCTTGACCATTTTTAGTG
AATGCAGGTGTTTTATTTGGAGGCTTGTGAATCTGGAAGTATTTTTGAAGGTCTTTTACC
AGAGGGTTTAAATATTTACGCGACAACTGCATCGAATGCAGAAGAAAGTAGCTGGGGTAC
TTACTGTCCTGGAGAGGATCCTAGTCCTCCTTCTGAGTATGAGACCTGTTTGGGTGACTT
ATACAGTGTTGCTTGGATCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCC
   
Sequence Name 19-K114804-0022-614-C01C-8409
GenBank Accession LN494876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114804-0022-614-C01C-8409
CAACACTGTATAAGTCACCCAAACAGGTCTCATACTCAGAAGGAGGACTAGGATCCTCTC
CAGGACAGTAAGTACCCCAGCTACTTTCTTCTGCATTCGATGCAGTTGTCGCGTAAATAT
TTAAACCCTCTGGTAAAAGACCTTCAAAAATACTTCCAGATTCACAAGCCTCCAAATAAA
ACACCTGCATTCACTAAAAATGGTCAAGCAAAATCACATGTAGCTGACAAAGAAAGCATT
TTCAAAAGAGCATTGTACCAAGCTCTTATATGTTCCTGAAGCATATTTTTTCTTCAAGAC
ATCATTGAGATCATTTGCATATAGGTTTGGAGAAGTTGGCATCCCTGAAAATAAACATGG
AGATTCAAATCATCAAAACTTTAGTAAAAAAATATATGAAAACTTCAACAAAATAGAGG

Last Updated on 06.12.2018 16:11