Sequences from confirmation amplicon


Confirmation sequences for line 614F02 and hit At1g08650 are shown.
Line ID 614F02
Locus At1g08650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2753143
Primers used for PCR BD29 - 8474, show primer details
   
Sequence Name 53-K028100-022-614-F02C-8409
GenBank Accession LN508036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K028100-022-614-F02C-8409
TACATTTGAACAAATTTGGTATTTTGTAATTTTGTTTTGTTGCAGGGCACCCATGGATCC
NAAGAGCAGGAGAAACAGAGGAGAGATTCATCTAGAAGATTCTCTCGGAGAAATAAATAA
GTGTCAAGTGATAGCTTATCATCTCTCTCGGTGGTTTTTTATCTAACAAGTATGGAGGAG
TGTCGGGCATGTAAATTTGGGTTATGGGCATTTTAGATTCCGCCAGCCTACCAACCTAGT
AGAAGAGAAGACTCTGGAAAAGACTTTTAACCAAAAAAAAAGGAGCTGAATAATAAAAGA
AGCTTGTTTGTTGTGGCTGCTTCTGAGTTTGGCGATGAGTTTTTTTTTTTTTGGCCCTTT
TGTTGCTAAGGAAAATTTAGTTTGGCT
   
Sequence Name 53-K028100-022-614-F02C-BD29
GenBank Accession LN508037 [GenBank]
Sequencing primer gene specific primer, BD29: 5'-AAAAGGCGGAAAAAGATTTGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K028100-022-614-F02C-BD29
GACTTTTCAAAGCCAAACAAAATTCACATAAGCAACAAATGCAGAAAAAAAAAAAAAACC
CATCCCCAAACTCAAAAGCCCCCCCAACAAACAAGCTTCTTTAATAATCCACCCCCTTTT
TTTTGGGTAAAAAGTCTTTTCCAAAGCCTTCCCTTCAACAAGGTGGGAAGGCGGGGGAAA
TCAAAAAGGCCCAAAACCCAAATTTACGGGCCCAACCCCCCCCCAAACTGGTAAAAAAAA
AACCCCCCGAGAGAAAGGAAAACCTACCACTGGACCCTTATTTATTTCCCCAAAAAAACC
TCCAAAAAAAACCCCCCCCCGGTTTCCCCGGCCCTTGGGACCCAGGGGGGCCCGGCAACA
AAACAAAATTACAAAAAACCAAATTTAGTTCAAATTGAAAAGGGCTCCGGGTCCGGAAAA
CCCCCGGGGATCACCAAGGAGTAGAAGGGTCAATAGGGAAAAAAAAAAAGAGTATTTCCC
ATTTTTTTTTCAATTCAAAAAGGAAAATGCCCCCCCCGTTTTTAAAAAAGGAAGGTACTT
TTTGAAAAAACGACAAATTACAACCCGCCGAATTTATGGGCAAAGGCAAAAAACAAATTA
TTCTAATTCGAAAATCTTTATTTCAACGGGCCCAC

Last Updated on 06.12.2018 16:11