Sequences from confirmation amplicon


Confirmation sequences for line 614F06 and hit At3g14520 are shown.
Line ID 614F06
Locus At3g14520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4876215
Primers used for PCR 25ze - 8474, show primer details
   
Sequence Name 29-K124164-0022-614-F06C-25ze
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 25ze: 5'-CATGGTTGTTGTGGATGACACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K124164-0022-614-F06C-25ze
GCGGTTCTTCCGAATGTTTGCAGAGGTGCTTGCTATATTTATGTGTTTATTCATAAATAT
ATGCCTCTTTCTTCTTCTTTATTTATTTATTCAATCGTGTAAAAATCTTGGCATGACCAG
GTTGAATATTGGTGCCGATGATAAACTTCCGGACTATTTACGAACAGTCCTCGAAAGTGT
GTTTGAAGTTATGGGAGAGATTGAACAAGAAATGAGACCGAAAGGACGATCATACGGCGT
GAAACAAGTGTTGGAAAGGGTAATGTAGCCAAATAAGCTGAATATGAAGCCAATTAATGT
ATATGTATAGTTAGTGGAATCTAATTAGTTCTTGGATTCTAAAACACAGTTCAAGAACGT
AGCAAAGGCGGACAAACAGTTAACAGAGTGGGCACGAACGGGCGACGTACCATCCTTTGA
TGAGTACATGAAAGTTGGGTTGGTGACAGCTGGGATGGATGGTTATGCAGGATATTGCTT
TATAGGAATGGAAGATGTCAGTGAAAAGGAAGCTTTTGAGTGGCTCAGTTCCAATCCCCT
AATCATCCAAGCGCTAAATGTAATGTTCCGTTTAGCAAATGACGTAGGAACCTACGAGGT
ACACGGCACATTAAATATAGGGATGTCTTTGGTTTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K124163-0022-614-F06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K124163-0022-614-F06C-8409
GCCATTTCAATTGAAAACCAAAGACATCCCTATATTTAATGTGCCGTGTACCTCGTAGGT
TCCTACGTCATTTGCTAAACGGAACATTACATTTAGCGCTTGGATGATTAGGGGATTGGA
ACTGAGCCACTCAAAAGCTTCCTTTTCACTGACATCTTCCATTCCTATAAAGCAATATCC
TGCATAACCATCCATCCCAGCTGTCACCAACCCAACTTTCATGTACTCATCAAAGGATGG
TACGTCGCCCGTTCGTGCCCACTCTGTTAACTGTTTGTCCGCCTTTGCTACGTTCTTGAA
CTGTGTTTTAGAATCCAAGAACTAATTAGATTCCACTAACTATACATATACATTAATTGG
CTTCATATTCAGCTTATTTGGCTACATTACCCTTTCCAACACTTGTTTCACGCCGTATGA
TCGTCCTTTCGGTCTCATTTCTTGTTCAATCTCTCCCATAACTTCAAACACACTTTCGAG
GACTGTTCGTAAATAGTCCGGAAGTTTATCATCGGCACCAATATTCAACCTGGTCATGCC
AAGATTTTTACACGATTGAATAAATAAATAAAGAAGAAGAAAGAGGCATATATTTATGAA
TAAACACATAAATATAGCAAGCACCTCTGCAAACATTCGGTAAGAACCGCAACTTCGGGT
ACAGTTGCATGTGCATCATAAGTGTCATCCACAACCAACCAT

Last Updated on 06.12.2018 16:11