Sequences from confirmation amplicon


Confirmation sequences for line 615B05 and hit At1g68460 are shown.
Line ID 615B05
Locus At1g68460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25668990
Primers used for PCR 07af - 8474, show primer details
   
Sequence Name 04-K106611-0022-615-B05C-8409
GenBank Accession LN487932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106611-0022-615-B05C-8409
TTACATTGAATGCCGCTTCACAATCTTTACGCTTGGCTCCAACACTTGCTCTTCCCAATT
CTCTCTCCACTTCTTCTCCGACGAACTCTTCATCATCACTGCTTTAAACGACGCCGTTGC
ATCAACTCTTTCTATTTCCCAACCAGCGTCTTTTAGCATCTCAATCTTCTTCACTTGTCT
CTTCGCTAACGTCCACGTGTTCCTTTTGATATCATCAACCGCCTTATCGTACGCCGCTTT
TCTTAAAGCGTCCCACTTTATCATCTTCTTCTCCGGTGGATACTCTTTGAAGTAACCGTC
AAACTCCGGTACACCTATAGCTTTCCTAATCCCAAACCGGGTTTCTAAACCGGATTTAAC
CGGGTCGTAGAATCTAGACAGCTCTTCGAACATACCTGAATCCATCATTTCG
   
Sequence Name 20-K106612-0022-615-B05C-07af
GenBank Accession LN495226 [GenBank]
Sequencing primer gene specific primer, 07af: 5'-ACGAAATGATGGATTCAGGTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K106612-0022-615-B05C-07af
CCGGTAATCCGGTTAGAAACCCGGTTTGGGATTAGGAAAGCTATAGGTGTACCGGAGTTT
GACGGTTACTTCAAAGAGTATCCACCGGAGAAGAAGATGATAAAGTGGGACGCTTTAAGA
AAAGCGGCGTACGATAAGGCGGTTGATGATATCAAAAGGAACACGTGGACGTTAGCGAAG
AGACAAGTGAAGAAGATTGAGATGCTAAAAGACGCTGGTTGGGAAATAGAAAGAGTTGAT
GCAACGGCGTCGTTTAAAGCAGTGATGATGAAGAGTTCGTCGGAGAAGAAGTGGAGAGAG
AATTGGGAAGAGCAAGTGTTGGAGCCAAGCGTAAAGATTGTGAAGCGGCATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCACGGT
TATT

Last Updated on 06.12.2018 16:11