Sequences from confirmation amplicon


Confirmation sequences for line 615F11 and hit At2g23070 are shown.
Line ID 615F11
Locus At2g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9826851
Primers used for PCR CD21 - 8474, show primer details
   
Sequence Name 19-K029489-022-615-F11C-8409
GenBank Accession LN494631 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K029489-022-615-F11C-8409
GCCATTGTTGGCCTTAATGTCTTTTTTTAACTCTTATTCAGAAAATGAAAAGTGAAGGTA
ACGAATTTGCTTTATTTCTCTCCTTTCTTTTTCTTTGGCTTCTCTTCGAAACGATGTCGT
TTAAACAATTCGGTCCGGTTATAGTGTGATTGAAATCGATCCGATTGGGTTTGTTTCGGT
TCGAGTGTGGTTGAATCGGTTCGATTTGGTTTAGGTGGTTTTTGGGTATCAGAGATTCTT
CTTCAACCTTTTTCTAATCCTGCACAACGCACTTCTTCTGTTGTCATCATCATCGTCCAC
TTGAAACAAACCTGACGATCAAAGAGAAGATTGCGATCGCAAATTTCTCCATTTTTTCAG
ATCGAAATTGATGTCGAAAGCTAGGGTTTATACAGATGTTAACGTTGTTCGTCCTAAAGA
GTATTGGGACTACGAGTCTCTCGTTGTTCAATGGGGGTAAAACTTCTAGCTTTAGAATTT
TTCTTTGATGAATTTACGATTTTTGACTTCTTTTTGATAAA
   
Sequence Name 20-K029489-022-615-F11C-CD21
GenBank Accession LN495084 [GenBank]
Sequencing primer gene specific primer, CD21: 5'-TTCCACCGAAAATTTATCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K029489-022-615-F11C-CD21
TCATCAAGAAATTCTAAAGCTAGAAGTTTTACCCCCATTGAACAACGAGAGACTCGTAGT
CCCAATACTCTTTAGGACGAACAACGTTAACATCTGTATAAACCNCTAGCTTTCGACATC
AATTTCGATCTGAAAAAATGGAGAAATTTGCGATCGCAATCTTCTCTTTGATCGTCAGGT
TTGTTTCAAGTGGACGATGATGATGACAACAGAAGAAGTGCGTTGTGCAGGATTAGAAAA
AGGTTGAAGAAGAATCTCTGATACCCAAAAACCACCTAAACCAAATCGAACCGATTCAAC
CACACTCGAACCGAAACAAACCCAATCGGATCGATTTCAATCACACTATAACCGGACCGA
ATTGTTTAAACGACATCGTTTCGAAGAGAAGCCAAAGAAAAAGAAAGGAGAGAAATAAAG
CAAATTCGTTACCTTCACTTTTCATTTTCTGAATAAGAGTTAAAAAAAGACATTAATGGC
CAACAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
A
Primers used for PCR FN51 - 8474, show primer details
   
Sequence Name 26-K035335-022-615-F11C-FN51
GenBank Accession LN497825 [GenBank]
Sequencing primer gene specific primer, FN51: 5'-CACATAGACCAGCAATTTTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K035335-022-615-F11C-FN51
CAGATACAATACAACAAATCAGATACCAATTCTGCATTACTTACGAGATTATATCATCAA
TCAAAAATATGAAACGAAACCCTAAATCTACATTCCACATTGATTCCAGAACAACCAATC
CCAAACCAATCTATAACCAAAGGCAAACATCTAGAAATCGACGTAATTAGGTTTAATTAG
AGAAGAATCTGAAGATATACCCCCATTGAACAGCAAGGGACTCGTAATCCCAATAGTCCT
TAGGTCTAACAACGTTGACATCAGCGTAAACCCTAGCTTTCGACGGCGCACCGGCACGCC
GGATAGATTTACCGATCTTCTGAGCCAGCGTTTCTGATTTTTCTTTCACGCGCGATTGCT
GTTGTTGCTGATGTTGTTGTTGTTGATTCCGGAGATGTTGACGGTAAAGAGAAGCGCTGG
ATGCGAAACGACGGAAATTGTCCTGGAGCGAAGAGAGAAGAAGATTCCTCTTCGCCGGAG
ACGACGAAGAGAAGGAGAGGAGAGAGAAGAGATTGTTGTTGGCGGCGGAAGCGTTGCGAA
GAGAGGAGATTGTGAATCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAGATCTTTATNTCGACGTGTCTACATTCACGCCCCAATGGG
   
Sequence Name 26-K035335-022-615-F11C-8409
GenBank Accession LN497824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K035335-022-615-F11C-8409
TCCGGAATGCAGCCATTTAAATTGAATATACATGGATTCACAATCTCCTCTCTTCGCAAC
GCTTCCGCCGCCAACAACAATCTCTTCTCTCTCCTCTCCTTCTCTTCGTCGTCTCCGGCG
AAGAGGAATCTTCTTCTCTCTTCGCTCCAGGACAATCTCCGTCGTTTCGCATCCAGCGCT
TCTCTTTACCGTCAACATCTCCGGAATCAACAACAACAACATCAGCAACAACAGCAATCG
CGCGTGAAAGAAAAATCAGAAACGCTGGCTCAGAAGATCGGTAAATCTATCCGGCGTGCC
GGTGCGCCGTCGAAAGCTAGGGTTTACGCTGATGTCAACGTTGTTAGACCTAAGGACTAT
TGGGATTACGAGTCCCTTGCTGTTCAATGGGGGTATATCTTCAGATTCTTCTCTAATTAA
ACCTAATTACGTCGATTTCTAGATGTTTGCCTTTGGTTATAGATTGGTTTGGGATTGGTT
GTTCTGGAATCAATGTGGAATGTAGATTTAGGGTTTCGTTTCATATTTTTGATTGATGAT
ATAATCTCGTAAGTAATGCAGAATTGGTATCTGATTTGTTTGTATTGGTATCTGTTTTGT
TTGTATATAATCTCTTGAAAATTGCTGGTCTATGTG

Last Updated on 06.12.2018 16:11