Sequences from confirmation amplicon


Confirmation sequences for line 616G02 and hit At3g27540 are shown.
Line ID 616G02
Locus At3g27540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10206128
Primers used for PCR CH76 - 8474, show primer details
   
Sequence Name 08-K107031-0022-616-G02C-8409
GenBank Accession LN489753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107031-0022-616-G02C-8409
TCGGACTGAGCCTTTACAATTGAAGCCATGCTGATCCATTGCTGATCTTTTAACGGTTGT
GATTCTTGAATCTAATTCTACGTTTACTGGTTTGCCTAAACCATTGGTGTTTAAGAGTAA
TAAGGATCAGTTTAAGTTTGTGGAGCCGCGGCTAACTTATGGGACGATTGGTGGACGGTT
TAGGAAAGGCGAGAATCCGTTTGTCGAAGAAGCGTATCAGCGAGTTGCGTTGGATCAGTT
GCTTAGGATTGCTGGTATTCAAGAAGATGATTTGTTGATAATGTCTGATGTTGATGAGAT
TCCTAGTGCTCATACTATCAATCTTTTGAGATGGTGTGATGATATTCCTCCAGTTCTTCA
CCTTCAGTTGAAGAATTATTTGTATTCTTTCGAATACTATGTCGATAGCAAGAGCTGGAG
AGCGTCTATACACCGTTACAGTCCAGGGAAAACGCGGTATGCTCATTTCCGTCAGAGTAA
TGTTATGTTAGCTGATTCTGGATGGCATTGTAGCTTCTGTTTCCGTTATATAAGTGAGTT
TATATTCAAGATGAAAGCTTATAGTCATTCAGACCGTGTCAGATTCTCTCATTACCTGAA
TCCAAGGAG
   
Sequence Name 24-K107032-0022-616-G02C-CH76
GenBank Accession LN497013 [GenBank]
Sequencing primer gene specific primer, CH76: 5'-CTCCTTGGATTCAGGTAATGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K107032-0022-616-G02C-CH76
AGCTTTCATCTTGAATAAAACTCACTTATATAACGGAAACAGAAGCTACAATGCCATCCA
GAATCAGCTAACATAACATTACTCTGACGGAAATGAGCATACCGCGTTTTCCCTGGACTG
TAACGGTGTATAGACGCTCTCCAGCTCTTGCTATCGACATAGTATTCGAAAGAATACAAA
TAATTCTTCAACTGAAGGTGAAGAACTGGAGGAATATCATCACACCATCTCAAAAGATTG
ATAGTATGAGCACTAGGAATCTCATCAACATCAGACATTATCAACAAATCATCTTCTTGA
ATACCAGCAATCCTAAGCAACTGATCCAACGCAACTCGCTGATACGCTTCTTCGACAAAC
GGATTCTCGCCTTTCCTAAACCGTCCACCAATCGTCCCATAAGTTAGCCGCGGCTCCACA
AACTTAAACTGATCCTTATTACTCTTAAACACCAATGGTTTAGGCAAACCAGTAAACGTA
GAATTAGATTCAAGAATCACAACCGTTAAAAGATCAGCAATGGATCAGCATGGCTTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11