Sequences from confirmation amplicon


Confirmation sequences for line 617C12 and hit At3g23727 are shown.
Line ID 617C12
Locus At3g23727 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8545293
Primers used for PCR 07rc - 8474, show primer details
   
Sequence Name 46-K115781-0022-617-C12C-07rc
GenBank Accession LN505963 [GenBank]
Sequencing primer gene specific primer, 07rc: 5'-TTCAAACGCCATCTTCAATAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115781-0022-617-C12C-07rc
AGTTTATGACTTACATAGTTAAATAAATCCCATAAAGAGTAAAAGATCCGATGATACAAG
AAAAACTGAACAACAAAATGATCGACCGAAGCATAAACATAATACATTTTGAACATATAT
TAGAAAACAAAAATAACTATATTTGAAAAGAGAAACAAATCAAAATAGACAGACATACAT
ATTGTCCTTTATCTCTTATTTTTGGCAAATATATTTTTATCCGAACCTATTGATTGCAAG
GAAGTTTCATATAACATATGCAGCGATGGTGGTTTTGCTTTCGCCCTTTTGTTTTATTAC
GTGTTGACAATCGCAACACCATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 22-K115780-0022-617-C12C-8409
GenBank Accession LN496254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115780-0022-617-C12C-8409
GATTGTCAACACGTAATAAAACAAAAGGGCGAAAGCAAAACCACCATCGCTGCATATGTT
ATATGAAACTTCCTTGCAATCAATAGGTTCGGATAAAAATATATTTGCCAAAAATAAGAG
ATAAAGGACAATATGTATGTCTGTCTATTTTGATTTGTTTCTCTTTTCAAATATAGTTAT
TTTTGTTTTCTAATATATGTTCAAAATGTATTATGTTTATGCTTCGGTCGATCATTTTGT
TGTTCAGTTTTTCTTGTATCATCGGATCTTTTACTCTTTATGGGATTTATTTAACTATGT
AAGTCATAAAACTCTAGTGATAAACTGATAATTATATACCTTTTAGTTATTGAAGATGGC
CGTTTGA

Last Updated on 06.12.2018 16:11