Sequences from confirmation amplicon


Confirmation sequences for line 618A06 and hit At5g42880 are shown.
Line ID 618A06
Locus At5g42880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17191823
Primers used for PCR NF42 - 8474, show primer details
   
Sequence Name 13-K102498-0022-618-A06C-8409
GenBank Accession LN491985 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K102498-0022-618-A06C-8409
TTTACAATTGATGAAGAAGGTCCAACGTCTTTCAATGACATCGACTTCTTGACATTCGAC
GATGAAGATTTACATAATCCATTTCAAGATTGTGAGACAAGTCCTATCAACGAACCTCCT
AAGGTTTATGTTGCACCAAGGGTGATGATAAGTCATCAAGATTCGTTTTCTGAAGATTCA
AGAACCGATGTTATCGAAGATGCTAGGATTTTGCCTGCTTCTCCTCGTCTTAGAGTTCCT
GCTTCTCCTCGAGCATTTGTGTATCCGAGATCTGTCGAATCACCTCGTTTTGGTTCTCCG
AGATCTGTCGAATCGCCTTGTTTCGGGTCTCCGATTGGTGTTATCGATACAGCTTCACCT
TTCGAATCGGTTAGAGAAGCAGTTTCTAAGTTTGGTGGGATCACAGATTGGAAAGCTCAT
AAGATTCAGACTATAGAGGTTGTAGTTAAGCTTTTGTTTCTTCACATGATTTGTGTTTAT
TTGAGATCTTGGTTTTGACGAAAATGTGTGTTTCAGAGACGGAAGATGGT
   
Sequence Name 13-K102499-0022-618-A06C-NF42
GenBank Accession LN491986 [GenBank]
Sequencing primer gene specific primer, NF42: 5'-ATCTACCATCTTCCGTCTCTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K102499-0022-618-A06C-NF42
CAATAAACCAAATCATGTGAAGAAACACAAGCTTAACTACACACCTCTATAGTCTGAATC
TTATGAGCTTTCCAATCTGTGATCCCACCAAACTTAGAAACTGCTTCTCTAACCGATTCG
AAAGGTGAAGCTGTATCGATAACACCAATCGGAGACCCGAAACAAGGCGATTCGACAGAT
CTCGGAGAACCAAAACGAGGTGATTCGACAGATCTCGGATACACAAATGCTCGAGGAGAA
GCAGGAACTCTAAGACGAGGAGAAGCAGGCAAAATCCTAGCATCTTCGATAACATCGGTT
CTTGAATCTTCAGAAAACGAATCTTGATGACTTATCATCACCCTTGGTGCAACATAAACC
TTAGGAGGTTCGTTGATAGGACTTGTCTCACAATCTTGAAATGGATTATGTAAATCTTCA
TCGTCGAATGTCAAGAAGTCGATGTCATTGAAAGACGTTGGACCTTCTTCATCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11