Sequences from confirmation amplicon


Confirmation sequences for line 618E10 and hit At3g59320 are shown.
Line ID 618E10
Locus At3g59320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21925755
Primers used for PCR MZ34 - 8474, show primer details
   
Sequence Name 07-K024137-022-618-E10C-MZ34
GenBank Accession LN489089 [GenBank]
Sequencing primer gene specific primer, MZ34: 5'-TTGAACCATTATTCTGAGAGGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024137-022-618-E10C-MZ34
TTACAAAAGATGTTACAGTTTCAGAGACATTAACATGGAAAAGAGAGTATTGATCGTATG
GTATCAGTTTTGTCACATACCTTGATTAAGATTGTGATTAATGTGTAAAACAGAAATAAC
CCGAGTGCGCCTCCAATGTAAAGTAAAATCTGGAACCACAAATTAATTTTCCAAAGAAGT
CATTCTATTTGCAGAGATTGAAAGGGAGTGTAGCAGCATGCTTACCGCCTCAGTTGACCA
CTGAATAGCTCTGACTGCACCACGTTCAAATATGCTTCTATGAACAAGTCAGGGAAAATG
TTCATAGAAGTTTAAGATAAATAAAAAAATAATTGCCTTGTTGTTTGAAGGATACATCTG
AATGGCAGCAATAATTGCGCCGAAAAGGCCCAAAAAAGCCATGAGCTCAGTCACGTCTGC
ATTCTTCACTAGGAACTCCTATAACAGAGAAACAGGGAGACTACAATTACAGTCACACTA
ACTTTCATAAAGTATCGACTTTCGCGATATAATAACAATCTTCTTTCCAGTATGTGGTTA
CATACTTGCTTACCTCAGTGACATTACTGACAGCATACAAGAAAATATGGAGAAAAAGAA
AGAGTAAAAGAAAGAAAAAGAAAGAAAAAGAAACTGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGG
   
Sequence Name 08-K024137-022-618-E10C-8409
GenBank Accession LN489553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024137-022-618-E10C-8409
CATGTAGATTTCAGTTTCTTTTTCTTTCTTTTTCTTTCTTTTACTCTTTCTTTTTCTCCA
TATTTTCTTGTATGCTGTCAGTAATGTCACTGAGGTAAGCAAGTATGTAACCACATACTG
GAAAGAAGATTGTTATTATATCGCGAAAGTCGATACTTTATGAAAGTTAGTGTGACTGTA
ATTGTAGTCTCCCTGTTTCTCTGTTATAGGAGTTCCTAGTGAAGAATGCAGACGTGACTG
AGCTCATGGCTTTTTTGGGCCTTTTCGGCGCAATTATTGCTGCCATTCAGATGTATCCTT
CAAACAACAAGGCAATTATTTTTTTATTTATCTTAAACTTCTATGAACATTTTCCCTGAC
TTGTTCATAGAAGCATATTTGAACGTGGTGCAGTCAGAGCTATTCAGTGGTCAACTGAGG
CGGTAAGCATGCTGCTACACTCCCTTTCAATCTCTGCAAATAGAATGACTTCTTTGGAAA
ATTAATTTGTGGTTCCAGATTTTACTTTACATTGGAGGCGCACTCGGGTTATTTCTGTTT
TACACATTAATCACAATCTTAATCAAGGTATGTGACAAAACTGATACCATACGATCAATA
CTCTCTTTTCCATGTTAATGTCTCTGAAACTGTAACATCTTTTGTAATGTGACCTC

Last Updated on 06.12.2018 16:11