Sequences from confirmation amplicon


Confirmation sequences for line 619F02 and hit At5g41770 are shown.
Line ID 619F02
Locus At5g41770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16720776
Primers used for PCR 07bc - 8474, show primer details
   
Sequence Name 45-K107114-0022-619-F02C-oriL
GenBank Accession LN505545 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K107114-0022-619-F02C-oriL
TTACATCGAAAGATATTAAAAATCGAGATATTATATATACGCAATGCGTACTCTGTTGAC
CCGTCTTCTCTAGTGATCGCCTTTCTTTTCTTGAGCTTCTTTGGAAGCTTCGATTGAACA
ATACTAACATCCCCGAGGTTACCAAAGCTACTCTCCATGTTCAGCCAATCCTCCAAGAGC
GTAGCTCGTTCTTCCTTCAGCTCTGGTGTGGAGTCTTTGTAATATGTGTTGGCTCTATCG
AAAATTGCTGAGTAGAAGAAATATAGAGAGAATAAACAGTTATAACATGTTGCAAGGAAT
CATCAAAACTGATATAATGTGTAGTTTTAAAGTTACTNNTGGCACG
   
Sequence Name 61-K107115-0022-619-F02C-07bc
GenBank Accession LN510534 [GenBank]
Sequencing primer gene specific primer, 07bc: 5'-ACGTGCCAGAGGTAACTTTAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K107115-0022-619-F02C-07bc
ACATGTTATAACTGTTTATTCTCTCTATATTTCTTCTACTCAGCAATTTTCGATAGAGCC
AACACATATTACAAAGACTCCACACCAGAGCTGAAGGAAGAACGAGCTACGCTCTTGGAG
GATTGGCTGAACATGGAGAGTAGCTTTGGTAACCTCGGGGATGTTAGTATTGTTCAATCG
AAGCTTCCAAAGAAGCTCAAGAAAAGAAAGGCGATCACTAGAGAAGACGGGTCAACAGAG
TACGCATTGCGTATATATAATATCTCGATTTTTAATATCTTTCGATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 44bc - 8474, show primer details
   
Sequence Name 44-K107114-0022-619-F02C-8409
GenBank Accession LN505200 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107114-0022-619-F02C-8409
CCATTTACAATCGAAAGATATTAAAAATCGAGATATTATATATACGCAATGCGTACTCTG
TTGACCCGTCTTCTCTAGTGATCGCCTTTCTTTTCTTGAGCTTCTTTGGAAGCTTCGATT
GAACAATACTAACATCCCCGAGGTTACCAAAGCTACTCTCCATGTTCAGCCAATCCTCCA
AGAGCGTAGCTCGTTCTTCCTTCAGCTCTGGTGTGGAGTCTTTGTAATATGTGTTGGCTC
TATCGAAAATTGCTGAGTAGAAGAAATATAGAGAGAATAAACAGTTATAACATGTTGCAA
GGAATCATCAAAACTGATATAATGTGTAGTTTTAAAGTTACCTCTGGCACGTTTGATGCA
ATCTTTCTTGTGTTCAATAACATCTTCTTCCTGGTCTTCATCTTCATTCTCGTCTTCCTC
TAGTTCCGCAGCAGAAGCTTCAAACTTTGCAAAGCTAACCCACACCTTGTATGGCTTNAG
TACG
   
Sequence Name 60-K107115-0022-619-F02C-44bc
GenBank Accession LN510274 [GenBank]
Sequencing primer gene specific primer, 44bc: 5'-CGTACTAAGCATTACAAGGTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107115-0022-619-F02C-44bc
GCTGCGGAACAGAGGAAACGAGAATGAAGATGAAGACCAGGAAGAAGATGTTATTGAACA
CAAGAAAGATTGCATCAAACGTGCCAGAGGTAACTTTAAAACTACACATTATATCAGTTT
TGATGATTCCTTGCAACATGTTATAACTGTTTATTCTCTCTATATTTCTTCTACTCAGCA
ATTTTCGATAGAGCCAACACATATTACAAAGACTCCACACCAGAGCTGAAGGAAGAACGA
GCTACGCTCTTGGAGGATTGGCTGAACATGGAGAGTAGCTTTGGTAACCTCGGGGATGTT
AGTATTGTTCAATCGAAGCTTCCAAAGAAGCTCAAGAAAAGAAAGGCGATCACTAGAGAA
GACGGGTCAACAGAGTACGCATTGCGTATATATAATATCTCGATTTTTAATATCTTTCGA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
Primers used for PCR CF85 - 8474, show primer details
   
Sequence Name 62-K118471-0022-619-F02C-CF85
GenBank Accession LN510856 [GenBank]
Sequencing primer gene specific primer, CF85: 5'-CACAAACCGAGAGATCAGAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118471-0022-619-F02C-CF85
CGAACCAAACAAAACATAACAGGGACAATTTTGAGTTTTGTTAGGTTCTTGGATAACAAA
AATTACTGCTAAACCAAACGATGTGTACAAAGAGCTAAGGAAGAATACAAAACACATTTC
ACAGTTTTGACTTTTGATATAACTTAAGAAAAAAGCTTAATCTCAATCATCCTCAGAAGC
AGCAACCTTCTGCTTCTTCCATTTGTATGCAGCTTCAAGAATCTTGAGATTCGTTGTTTG
CGATTCTTCTGGGTATAAATAATCGATGTATTCTTCGTACCTGTTTAAACATTCAAACAA
TTTTTCACGTTAAGCTTTAGCATGAGTATGGCTTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 30-K118470-0022-619-F02C-8409
GenBank Accession LN499920 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118470-0022-619-F02C-8409
ACTCATGCTAAAGCTTAACGTGAAAAATTGTTTGAATGTTTAAACAGGTACGAAGAATAC
ATCGATTATTTATACCCAGAAGAATCGCAAACAACGAATCTCAAGATTCTTGAAGCTGCA
TACAAATGGAAGAAGCAGAAGGTTGCTGCTTCTGAGGATGATTGAGATTAAGCTTTTTTC
TTAAGTTATATCAAAAGTCAAAACTGTGAAATGTGTTTTGTATTCTTCCTTAGCTCTTTG
TACACATCGTTTGGTTTAGCAGTAATTTTTGTTATCCAAGAACCTAACAAAACTCAAATT
TGTCCTGTTATGTTTTGTTTTGGTTCGAATTTTCCGGTTTAGAAATTAATCAGGATACTT
CTTCATTCCGGTTTATGTTCTGATCTCCGGGGGTTTGTG
Primers used for PCR 17mg - 8474, show primer details
   
Sequence Name 63-K118471-0022-619-F02C-17mg
GenBank Accession LN511085 [GenBank]
Sequencing primer gene specific primer, 17mg: 5'-ACTAAGCATTACAAGGTGTGGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118471-0022-619-F02C-17mg
CAGGAAGAAGAAGTTATTGAACACAAGAAAGATTGCATCAAACGTGCCAGAGGTAACTTT
AAAACTACACATTATATCAGTTTTGATGATTCCTTGCAACATGTTATAACTGTTTATTCT
CTCTATATTTCTTCTACTCAGCAATTTTCGATAGAGCCAACACATATTACAAAGACTCCA
CACCAGAGCTGAAGGAAGAACGAGCTACGCTCTTGGAGGATTGGCTGAACATGGAGAGTA
GCTTTGGTAACCTCGGGGATGTTAGTATTGTTCAATCGAAGCTTCCAAAGAAGCTCAAGA
AAAGAAAGGCGATCACTAGAGAAGACGGGTCAACAGAGTACGCATTGCGTATATATAATA
TCTCGATTTTTAATATCTTTCGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCC
   
Sequence Name 31-K118470-0022-619-F02C-oriL
GenBank Accession LN500346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118470-0022-619-F02C-oriL
ATTTACATCGAAAGATATTAAAAATCGAGATATTATATATACGCAATGCGTACTCTGTTG
ACCCGTCTTCTCTAGTGATCGCCTTTCTTTTCTTGAGCTTCTTTGGAAGCTTCGATTGAA
CAATACTAACATCCCCGAGGTTACCAAAGCTACTCTCCATGTTCAGCCAATCCTCCAAGA
GCGTAGCTCGTTCTTCCTTCAGCTCTGGTGTGGAGTCTTTGTAATATGTGTTGGCTCTAT
CGAAAATTGCTGAGTAGAAGAAATATAGAGAGAATAAACAGTTATAACATGTTGCAAGGA
ATCATCAAAACTGATATAATGTGTAGTTTTAAAGTTACCTCTGGCACGTTTGATGCAATC
TTTCTTGTGTTCAATAACATCTTCTTCCTGGTCTTCATCTTCATTCTCGTCTTCCTCTAG
TTCCGCAGCAGAAGCTTCAAACTTTGCAAAGCTAACCCACACCT

Last Updated on 06.12.2018 16:11