Sequences from confirmation amplicon


Confirmation sequences for line 621F09 and hit At4g00480 are shown.
Line ID 621F09
Locus At4g00480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:219691
Primers used for PCR BD85 - 8474, show primer details
   
Sequence Name 23-K028348-022-621-F09C-8409
GenBank Accession LN496426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K028348-022-621-F09C-8409
CATTTAAATTGAAATATCCTGAAGAACTGAATTTAAAAGTATGGAACCAAAGTGATTGAT
AAAGAGGAGACAACCAAAAGGCAAAATTTATTAAAGAAGGGTTTGGTATTGAAGCTTTAT
TTCCTTTTCTTTCTTTCTTTATTGCTTTCTCTTTCTCAGCTTGGTCCTGATGGAAAGTCT
TAAAAGAGTGTCTTTAAAGTTGAGTACTGTGTCTGAATTATGAGAGAGAGTGGTTGACTT
GGGTCATGATCTTGGTTTTTGGAGTGACTTATCTTTATACAATACATATGGTAATAGTGT
TGGATTCTATTTGGTGAAAACCAAGGTAAAATATTCTCTAAATTAGTGATGATGCTGAAA
ATAATTGCA
   
Sequence Name 23-K028348-022-621-F09C-BD85
GenBank Accession LN496427 [GenBank]
Sequencing primer gene specific primer, BD85: 5'-TTTGCAATATTTTTCAGCATCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K028348-022-621-F09C-BD85
AAGCTTCAATACCAAACCCTTTCTTTAATAAATTTTAGCCTTTTAGGTTCGTCTCCTCTT
TATCAATCACTTTGGTTCCATACTTTTTAAATTTCAGTTCTTCAGGAATATATTCAATTC
GTAAATCGGCTTTCATCGTCCGGGAAAATCTACATCGGATCAGCAATGAGTATGTTGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCAATTTCAAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTTGATAAAACGACAAATTACGAATCC
GTCGTAATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTTATTTC
GACGTGTCTA

Last Updated on 06.12.2018 16:11