Sequences from confirmation amplicon


Confirmation sequences for line 622C01 and hit At1g15950 are shown.
Line ID 622C01
Locus At1g15950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5479398
Primers used for PCR AC13 - 8474, show primer details
   
Sequence Name 38-K117403-0022-622-C01C-AC13
GenBank Accession LN503131 [GenBank]
Sequencing primer gene specific primer, AC13: 5'-TCTCGAGAGAGGTTACACAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117403-0022-622-C01C-AC13
TTTATATATACTTTTTTGGTCACTTGTGATAATTATCTGAAGTTGTTGAGGTTATTTTTA
TTGTTTTGATTGACAATTTGGATTTCTTTATAATTATTTTTCTTGCAGATGATCCGAAGA
ACACACATTTGAGAGAACTAGAAGGAGGAAAGGAGAGACTGATTCTGTGCAAAGCAGATC
TTCAGGACTACGAGGCTCTTAAGGCGGCGATTGATGGTTGCGACGGCGTCTTTCACACGG
CTTCTCCTGTCACCGACGATCCGGTGAGTCTTTTTCTTGGGTTTTCTATTTGTTTTATCG
CATTTTTAGTGGCTTACAGAGAGAATCGAATAAAGTTAAGGACCTACATGAAATAAAATA
ATACTTATATAGTAGTGGAGCTGATGATTTCATTTAGATTTCATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCCACAAGTTTTTT
   
Sequence Name 14-K117402-0022-622-C01C-8409
GenBank Accession LN492690 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117402-0022-622-C01C-8409
TCTAAATGAAATCATCAGCTCCACTACTATATAACTATTATTTTATTTCATGTAGGTCCT
TAACTTTATTCGATTCTCTCTGTAAGCCACTAAAAATGCGATAAAACAAATAGAAAACCC
AAGAAAAAGACTCACCGGATCGTCGGTGACAGGAGAAGCCGTGTGAAAGACGCCGTCGCA
ACCATCAATCGCCGCCTTAAGAGCCTCGTAGTCCTGAAGATCTGCTTTGCACAGAATCAG
TCTCTCCTTTCCTCCTTCTAGTTCTCTCAAATGTGTGTTCTTCGGATCATCTGCAAGAAA
AATAATTATAAAGAAATCCAAATTGTCAATCAAAACAATAAAAATAACCTCAACAACTTC
AGATAATTATCACAAGTGACCAAAAAAGTATATATAAAAATGGACAAGTACCTGGATTCC
GTACGGTTCCTTTGACTGTGTAACCCTTCTCGAGA

Last Updated on 06.12.2018 16:11