Sequences from confirmation amplicon


Confirmation sequences for line 622E12 and hit At5g23820 are shown.
Line ID 622E12
Locus At5g23820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8031794
Primers used for PCR 40xc - 8474, show primer details
   
Sequence Name 52-K119175-0022-622-E12C-40xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40xc: 5'-AACCGGTTGTACTAGTTTTAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K119175-0022-622-E12C-40xc
TCCACCTTGGGTTTGAACATTGGTCCTAGGATTAAGGAAAATTCACATTCATTCATGGCT
TTTTATTTTCCTATGCTAAGAGTAGAAATGTAGTTACCTCATCATAACCAACATAAAGAA
CGTCGGAAAGAGTTAACTCAAAGTTGGTGCCAGCTTCAATCTCAGCTTCATTGTTACATG
CACTCACATCACAAAGGTTGTAAGTTTTTAAAAGGCCGGTAAATTCCCCATCTCTAAAGG
CTACGTACACAAGGGTTCCCGCGGGGATGTTTTTGCCTATATATATAAAGGATTTTATCA
AGAAAATATTCAAAACTAGAGAAAAAAAGAGAGAGTTGAAACTAGATCATAAAATTAATT
GTTACTTACTTGCAGAACCGAATAAATTTATAGTGATGGTTGGGTTTTCGTGAATGGCTA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGT
   
Sequence Name 24-K119174-0022-622-E12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119174-0022-622-E12C-8409
CATTTACATTGAATATAGCCATTCACGAAAACCCAACCATCACTATAAATTTATTCGGTT
CTGCAAGTAAGTAACAATTAATTTTATGATCTAGTTTCAACTCTCTCTTTTTTTCTCTAG
TTTTGAATATTTTCTTGATAAAATCCTTTATATATATAGGCAAAAACATCCCCGCGGGAA
CCCTTGTGTACGTAGCCTTTAGAGATGGGGAATTTACCGGCCTTTTAAAAACTTACAACC
TTTGTGATGTGAGTGCATGTAACAATGAAGCTGAGATTGAAGCTGGCACCAACTTTGAGT
TAACTCTTTCCGACGTTCTTTATGTTGGTTATGATGAGGTAACTACATTTCTACTCTTAG
CATAGGAAAATAAAAAGCATGAATGAATGTGAATTTTCCTTAATCCTAGGACCAATGTTT
CAAACCCAAGGTTGGACTAATTAAATAACCGTAATTGACCGGCAAAACCCCTAAAACTAG
TACAACCGGTTAAAANAAGAATGAGTAATTACCAATT

Last Updated on 06.12.2018 16:11