Sequences from confirmation amplicon


Confirmation sequences for line 622H06 and hit At5g18270 are shown.
Line ID 622H06
Locus At5g18270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6041511
Primers used for PCR CH84 - 8474, show primer details
   
Sequence Name 12-K107031-0022-622-H06C-8409
GenBank Accession LN491600 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107031-0022-622-H06C-8409
CGGACTGAGCCATTTACATTGAATATATCCTGCCATTTACCATCAATCTTTAACCACCAG
GTTTTTGGTTCTGCTTCGGGCTCTACGTACAACAACAACAACGAGATGATCAAGATGGAG
CAATCACTTGTTAGTGTATCTCAAGAAACATGCCTAAGCTCAGATGTGAACGCGAACATG
ACTACAACCACGGAGGTATCTTCGGGTCCTGTAATGAAACAAGAAATGGGGATGATGGGA
ATGGTGAATGGTAGCAAGTCGTATGAAGATCTATGTGACTTGAGGGGGGACTTGTGGGAC
TTCTAATTAATCATTTGACTGTGGTGAAAGAGTATATTTGTTGGGATTTAAATCATGTTA
GTTAATACATATACATATAGGATTTACTAGAGGCTTAATCCTAGTTAACTATTTTCACTT
CATTGATATTATTTAATTAGTTGATTGTTTAATTAGTTTATACTTTATAGTGTGGTTAAA
AAAGAAAAGAAAGGATTGTGATAATTTGGGA
   
Sequence Name 28-K107032-0022-622-H06C-CH84
GenBank Accession LN498868 [GenBank]
Sequencing primer gene specific primer, CH84: 5'-TCCCAAATTATCACAATCCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107032-0022-622-H06C-CH84
TAATTAAACATCAACTAATTAAATAATATCAATGAAGTGAAAATAGTTAACTAGGATTAA
GCCTCTAGTAAATCCTATATGTATATGTATTAACTAACATGATTTAAATCCCAACAAATA
TACTCTTTCACCACAGTCAAATGATTAATTAGAAGTCCCACAAGTCCCCCCTCAAGTCAC
ATAGATCTTCATACGACTTGCTACCATTCACCATTCCCATCATCCCCATTTCTTGTTTCA
TTACAGGACCCGAAGATACCTCCGTGGTTGTAGTCATGTTCGCGTTCACATCTGAGCTTA
GGCATGTTTCTTGAGATACACTAACAAGTGATTGCTCCATCTTGATCATCTCGTTGTTGT
TGTTGTACGTAGAGCCCGAAGCAGAACCAAAAACCTGGTGGTTAAAGATTGATTGGTAAA
TGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGGGTTATT

Last Updated on 06.12.2018 16:11