Sequences from confirmation amplicon


Confirmation sequences for line 625B02 and hit At2g27510 are shown.
Line ID 625B02
Locus At2g27510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11758516
Primers used for PCR 12ea - 8474, show primer details
   
Sequence Name 51-K110361-0022-625-B02C-12ea
GenBank Accession LN507534 [GenBank]
Sequencing primer gene specific primer, 12ea: 5'-CATCTTCTCATTACGCTCCATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K110361-0022-625-B02C-12ea
TTGCTTTGGAACAGATAAGTTTTTGGTTCTTGTTTCCTTGCTACTTGTGATGCACATCCT
CACAATTTTCTCTCAGTTTTGGTTCTTATTTCTCTGGAACAGTTTGATTTGTTAGATTGT
ATCACTATGAAGAAACCCTGAAGCTAAACTTGTTTATAAACGCAGGTGATAAACAAGAAT
GGCGACTGTACGAATCTCCTCCACTAGCATGACCAAGGCCGTTCTTAGAAGCCAGACAAC
CAACAAGCTCATTACTAACAAGAGCTACAACTTATCTGTTGGATCAACGAAAAGGGTTTC
GAGATCTTTTGGTTTGAAATGCTCAGCGAATTCTGGTGGTGCTACGATGTCGGCGGTATA
CAAGGTCAAGCTCTTAGGACCTGATGGTCAAGAAGATGAGTATGAGGTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 35-K110360-0022-625-B02C-8409
GenBank Accession LN501868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K110360-0022-625-B02C-8409
GCCATTTACAATTGAATATATCCTGAACCTCATACTCATCTTCTTGACCATCAGGTCCTA
AGAGCTTGACCTTGTATACCGCCGACATCGTAGCACCACCAGAATTCGCTGAGCATTTCA
AACCAAAAGATCTCGAAACCCTTTTCGTTGATCCAACAGATAAGTTGTAGCTCTTGTTAG
TAATGAGCTTGTTGGTTGTCTGGCTTCTAAGAACGGCCTTGGTCATGCTAGTGGAGGAGA
TTCGTACAGTCGCCATTCTTGTTTATCACCTGCGTTTATAAACAAGTTTAGCTTCAGGGT
TTCTTCATAGTGATACAATCTAACAAATCAAACTGTTCCAGAGAAATAAGAACCAAAACT
GAGAGAAAATTGTGAGGATGTGCATCACAAGTAGCAATGGAAACAAGAACCAAAAACTTA
TCTGTTTCCAATAGCAAATATGATGTGAAAATTAGTGTATGGAGCGTAATGAGAAGATG

Last Updated on 06.12.2018 16:11