Sequences from confirmation amplicon


Confirmation sequences for line 625G07 and hit At1g55510 are shown.
Line ID 625G07
Locus At1g55510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20723742
Primers used for PCR 12ht - 8474, show primer details
   
Sequence Name 43-K112586-0022-625-G07C-12ht
GenBank Accession LN504891 [GenBank]
Sequencing primer gene specific primer, 12ht: 5'-AGAATCTGTTTCTTTCTTCACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112586-0022-625-G07C-12ht
AATCTTACAGTTGGGTGAAGTTGCCAAATAGAGCTTGCTTTTGTCGCAATCCTATATTTT
TCAGATTGATTGTTGGTGGGTTTGTGTAAATGGCGGCTCTTTTAGGCAGATCCTGCCGGA
AACTGAGTTTTCCGAGCTTGAGTCACGGAGCTAGGAGGGTATCGACGGAAACTGGAAAAC
CATTGAATCTATACTCTGCTATTAATCAAGCGCTTCACATCGCTTTGGACACCGATCCTC
GGTAAGCTTACACTAGACATTTGATCCTTAGGTTAATAGACATCTAATACATTCTGTGGA
GAAAGATAAAAAAAATGTGGGGAATTTAGTTAATTAGTATACACATATAGCAGGATCCCC
AATTCCCATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K112585-0022-625-G07C-8409
GenBank Accession LN493919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112585-0022-625-G07C-8409
ATGGGGATCTGCTATTGTGTATCTAATTACTAAATTCCCCACATTTTTTTTATCTTTCTC
CACAGAATGTATTAGATGTCTATTAACCTAAGGATCAAATGTCTAGTGTAAGCTTACCGA
GGATCGGTGTCCAAAGCGATGTGAAGCGCTTGATTAATAGCAGAGTATAGATTCAATGGT
TTTCCAGTTTCCGTCGATACCCTCCTAGCTCCGTGACTCAAGCTCGGAAAACTCAGTTTC
CGGCAGGATCTGCCTAAAAGAGCCGCCATTTACACAAACCCACCAACAATCAATCTGAAA
AATATAGGATTGCGACAAAAGCAAGCTCTATTTTGGCAACTTTCACCCAACTGGTAAGAT
TTTTAGGTGGCAAAGGTTTGCTACTTTTGGTGGGTGAAGAAAGAAACAGATTC

Last Updated on 06.12.2018 16:11