Sequences from confirmation amplicon


Confirmation sequences for line 625H05 and hit At5g01020 are shown.
Line ID 625H05
Locus At5g01020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7879
Primers used for PCR 21ai - 8474, show primer details
   
Sequence Name 57-K106741-0022-625-H05C-21ai
GenBank Accession LN509430 [GenBank]
Sequencing primer gene specific primer, 21ai: 5'-TCAAGCTCAAGGTTTCTCTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K106741-0022-625-H05C-21ai
GTTCTTTTTTCTGATCTCCTCCTCTTTGGTTCCCTCGCAGCCCAACAGCTCCCGCTTTAA
AACTCCCGATCCGTCTCAGATCTGAGCGATCCATCCACTCCTCGCTTCCGAGACGACTCT
CGCACTCCTATTTCGTATGCCCAAGTCATCCCCTTTACTTTGTTTGAGCTTGAGACCATC
ACCAAGAGCTTCCGCCCAGATTATATACTCGGAGAAGGTGGTTTCGGTACCGTCTACAAG
GGTTACATTGATGACAATCTCCGCGTCGGCCTCAAGTCTCTCCCTGTTGCCGTCAAGGTC
CCAGTCGCTCAAACAATGTAAAGGGGAGAGAGATTGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGAAGCGTTATTAT
   
Sequence Name 41-K106740-0022-625-H05C-8409
GenBank Accession LN504091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K106740-0022-625-H05C-8409
TCGGACTGAGCCTTTACATTGAATATATCCATCTCTCTCCCCTTTACATTGTTTGACCGA
CTGGGACCTTGACGGCAACAGGGAGAGACTTGAGGCCGACGCGGAGATTGTCATCAATGT
AACCCTTGTAGACGGTACCGAAACCACCTTCTCCGAGTATATAATCTGGGCGGAAGCTCT
TGGTGATGGTCTCAAGCTCAAACAAAGTAAAGGGGATGACTTGGGCATACGAAATAGGAG
TGCGAGAGTCGTCTCGGAAGCGAGGAGTGGATGGATCGCTCAGATCTGAGACGGATCGGG
AGTGCTTCTTCTGGAGCTGTTGGGCTGCGAGGTGAACCAAAGAGGAGAGAGATCAGGAAA
AAAGAAACGGCGAGAGACAGAGATAGAGAGAAAGAGAAACCTTGAGCTTGA

Last Updated on 06.12.2018 16:11