Sequences from confirmation amplicon


Confirmation sequences for line 626D10 and hit At1g16310 are shown.
Line ID 626D10
Locus At1g16310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5578736
Primers used for PCR 86yh - 8474, show primer details
   
Sequence Name 45-K119974-0022-626-D10C-86yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 86yh: 5'-TTCTCACAACTTCCCCTAGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K119974-0022-626-D10C-86yh
AGCGATCGGATATGGCGACGGAGCAATAACTCGTACGGGAGATGAATACATGTCGAGCTT
TTACCTAGTGACGACGATGCGCCGCCGCTTGAATCGTCTTGGCGTCTCAACCTCGACGCT
TTTCAACTCCCTTCTTCCACCGGTGGCCGTCACGACGGACGTACTCGCTTTTCTCGCTAT
TTTCGTACTCCAAGTAATTTTTTTCTTTTTCTTCATGTTTTGAAGCCGTATTGACCTACA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGC
   
Sequence Name 13-K119973-0022-626-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119973-0022-626-D10C-8409
GCCATTTACATTGAATATATCCTGTAGGTCAATACGGGCTTCAAAACATGAAGAAAAAGA
AAAAAATTACTTGGAGTACGAAAATAGCGAGAAAAGCGAGTACGTCCGTCGTGACGGCCA
CCGGTGGAAGAAGGGAGTTGAAAAGCGTCGAGGTTGAGACGCCAAGACGATTCAAGCGGC
GGCGCATCGTCGTCACTAGGTAAAAGCTCGACATTGTATTCATCTCCCGTACGAGTTATA
TGCTCCGTCGCCATTATCCGATCGCTGTGCGTTCGAATTCCGAAAAAAGTGTTTCTAGGG
GAAGTTGTGAGAAACCCC

Last Updated on 06.12.2018 16:11