Sequences from confirmation amplicon


Confirmation sequences for line 626G07 and hit At4g32660 are shown.
Line ID 626G07
Locus At4g32660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15756922
Primers used for PCR 34qz - 8474, show primer details
   
Sequence Name 28-K115806-0022-626-G07C-34qz
GenBank Accession LN498988 [GenBank]
Sequencing primer gene specific primer, 34qz: 5'-AAGATAAATAAATACCGTGTGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115806-0022-626-G07C-34qz
CTGAGTCATCAATTTCAATCATTGCTGCATCCCGGTATTTCTTAATGCTTCGAATTATCT
TTATTGCCACATATTCTTTTGTATCACGATCCCAACATTCCAAAACCCGACCAAATGTGC
CTACAACACCAGCAAGTTCACTGAAATGGCACGATTTGATTCACATAAACATTTTTTTTA
TCTCGAACACACCATGCCACTTGACAGCTAACCTTCACCCATCTTGCTAAGTATTTTATC
TGTCAAAAGTCAAAACAGAACACACAATAGCGTAAGTTAAGCAATTAAAAATGTTCAGGA
AGGATTCTCAAATGTAATGAGTATATTCAATTGTAAGTGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
   
Sequence Name 07-K115805-0022-626-G07C-8409
GenBank Accession LN489417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115805-0022-626-G07C-8409
CCTTACATTGAATATCTCATTACATTTGAGAATCCTTCCTGAACATTTTTAATTGCTTAA
CTTACGCTATTGTGTGTTCTGTTTTGACTTTTGACAGATAAAATACTTAGCAAGATGGGT
GAAGGTTAGCTGTCAAGTGGCATGGTGTGTTCGAGATAAAAAAAATGTTTATGTGAATCA
AATCGTGCCATTTCAGTGAACTTGCTGGTGTTGTAGGCACATTTGGTCGGGTTTTGGAAT
GTTGGGATCGTGATACAAAAGAATATGTGGCAATAAAGATAATTCGAAGCATTAAGAAAT
ACCGGGATGCAGCAATGATTGAAATTGATGTACTTCAGAAACTTGTTAAGAGTGATAAAG
GCCGCACACGGTATTTATTTATCTTA

Last Updated on 06.12.2018 16:11