Sequences from confirmation amplicon


Confirmation sequences for line 626H10 and hit At3g10110 are shown.
Line ID 626H10
Locus At3g10110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3116868
Primers used for PCR PF76 - 8474, show primer details
   
Sequence Name 01-K103223-0022-626-H10C-8409
GenBank Accession LN486532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103223-0022-626-H10C-8409
GCAACAATGGGGCAAGGAGTTGGAATTCCTGTAAGACATTTGCAGTTATGGGTTTGGTTT
TCTCTGCTGCAGAATGCATTGTCGAAAAGGTAATACCTTGTTTATTGACCAAGAAAAACA
AGGAAATTCCACTACTAACTCATGTATCATAACTTCAAAACCTTATTATCGACTTTTTTG
GTTTACCTCTCTGAAAAAGGCAAGAGCTAAGCATGACACTGTAAACACCGCTNTAGCTGG
ATGTGTTACCGGTGGTTCAATGTCCGCCCGAGGTAAGACCTATTATCTTCTGTGCATGGA
ATTTAAATCTTAGAGATTCATTATCAATATGCAACGGCCTAATTCCCAAACTGTAATCTC
GTAGAGGTTTGATTTCTCTTACATGATACATTGTGTTCTTGAGGACTTAATGGATTGGTC
TTGTTTCAGGTGGGCCAAAAGCTGCGTGTATAGGTTGCGCTGGATTTGCAACTTTCTCTG
TACTCATAGAGAAGTTCTTTGATAGGCATACATAAACCAGTGAGATATCTAACAGACGCC
NTTGCTGAGATTTTTTTGCTGCTTCTTTTCTGTGATAGTGATACAGTTCATCACCTGAAC
CCAGGGAGATAGAAGCTAGAATTACACTTTTCAATATAGAAAAAGCAAGGCT
   
Sequence Name 49-K103230-0022-626-H10C-PF76
GenBank Accession LN506804 [GenBank]
Sequencing primer gene specific primer, PF76: 5'-ATCAACACAAGCCTTGCTTATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103230-0022-626-H10C-PF76
CTCCCTGGGTTCAGGTGATGAACTGTATCACTATCACAGAAAAGAAGCAGCAAAAAAATC
TCAGCAATGGCGTCTGTTAGATATCTCACTGGTTTATGTATGCCTATCAAAGAACTTCTC
TATGAGTACAGAGAAAGTTGCAAATCCAGCGCAACCTATACACGCAGCTTTTGGCCCACC
TGAAACAAGACCAATCCATTAAGTCCTCAAGAACACAATGTATCATGTAAGAGAAATCAA
ACCTCTACGAGATTACAGTTTGGGAATTAGGCCGTTGCATATTGATAATGAATCTCTAAG
ATTTAAATTCCATGCACAGAAGATAATAGGTCTTACCTCGGGCGGACATTGAACCACCGG
TAACACATCCNGCTATAGCGGTGTTTACAGTGTCATGCTTAGCTCTTGCCTTTTTCAGAG
AGGTAAACCAAAAAAGTCGATAATAAGGTTTTGAAGTTATGATACATGAGTTAGTAGTGG
AATTTCCTTGTTTTTCTTGGTCAATAAACAAGGTATTACCTTTTCGACAATGCATTCTGC
AGCAGAGAAAACCAAACCCATAACTGCAAATGTCTTACAGGAATTCCAACTCCTTTGCCC
CATTTGTTTTGCCGTGAACACAATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATNGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11