Sequences from confirmation amplicon


Confirmation sequences for line 630C04 and hit At5g37450 are shown.
Line ID 630C04
Locus At5g37450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14853932
Primers used for PCR EU24 - 8474, show primer details
   
Sequence Name 03-K103431-0022-630-C04C-EU24
GenBank Accession LN487450 [GenBank]
Sequencing primer gene specific primer, EU24: 5'-TTGCTTTCTTCCTTGTATTGTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103431-0022-630-C04C-EU24
GCAACGAAAGAGAAAACTAGGGAAGTCGACATGGAACAAGAACACCCACGTAAGTGGAAA
CTTAGTCAATTTCTCTGTTTTCCTTAAACGTATATCTAATGCTAATCTCCCATGTTTCTC
CTTGGCCTAGTTCCAAAACCTCCAATGAACATGGAGAGCGTGAAGGGTTACAATTTCACG
GAACTTGATTCAGCAACAAGCAGTTTCAGTGATCTCTCTCAGATTGGTAGAGGTGGCTAT
GGGAAGGTCTACAAAGGCCATTTACCAGGTGGTCTGAGATCTCGGTATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
T
   
Sequence Name 19-K103432-0022-630-C04C-8409
GenBank Accession LN494723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103432-0022-630-C04C-8409
TTACATTGAATACCGAGATCTCAGACCACCTGGTAAATGGCCTTTGTAGACCTTCCCATA
GCCACCTCTACCAATCTGAGAGAGATCACTGAAACTGCTTGTTGCTGAATCAAGTTCCGT
GAAATTGTAACCCTTCACGCTCTCCATGTTCATTGGAGGTTTTGGAACTAGGCCAAGGAG
AAACATGGGAGATTAGCATTAGATATACGTTTAAGGAAAACAGAGAAATTGACTAAGTTT
CCACTTACGTGGGTGTTCTTGTTCCATGTCGACTTCCCTAGTTTTTCTCTTTCGTTTGCT
TCTCTTGATGAAGAAGACCAATGCTAGAGAGGACAATACAAGGAAGAAAGCAAAC

Last Updated on 06.12.2018 16:11