Sequences from confirmation amplicon


Confirmation sequences for line 630G07 and hit At2g34920 are shown.
Line ID 630G07
Locus At2g34920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14729875
Primers used for PCR 30vh - 8474, show primer details
   
Sequence Name 79-K117751-0022-630-G07C-30vh
GenBank Accession LN513874 [GenBank]
Sequencing primer gene specific primer, 30vh: 5'-TCCGAGGCCTAGATATTTCTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K117751-0022-630-G07C-30vh
CATCCGCTTTGGAATGACATTTCTTGTGTCTCAAGGAACGGTGTCTCTTGAGTTCCCTCT
GTACCGCTTTCTAGTTTCCCCGCTTCCTCGTTCCTTAGTATCCGCGGTTCTTGAGGAGTT
ACGCTGGTGAGAGGACTAGTGTTATCAGATTCTCTTTCCAATACTTCTGCTACTATGGCT
TTTTTCAGCTGAAGGTAACTCATTTTGGTTTCAGTTTCGTTCTTGCGTAAGATTGCTTCC
TCTATGTTGCGTTTTTGAGGACTTAACCGCTCTGTAGAAGGAGAATGAAGAATACCACTG
GTTTCTTTTGACTGCATGCTCTCTGTTTCTACGGTGGATTGTTGACCCTTCTTTAGTCCG
GCTTCAGCGGCAGCGTTTGCTGCTTTTTCCCGGAGTTTTTCTCTGCATTATGATTTAAAC
ATTAAGGAGAGGAGAAAAATGAGTTCATGAACTTGTAGGACGTAAAACAGAGGTTCTTGA
CGTAAAACAGACGTAAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 54-K117750-0022-630-G07C-8409
GenBank Accession LN508612 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117750-0022-630-G07C-8409
TATCCTGTTTTACGTCTGTTTTACGTCAAGAACCTCTGTTTTACGTCCTACAAGTTCATG
AACTCATTTTTCTCCTCTCCTTAATGTTTAAATCATAATGCAGAGAAAAACTCCGGGAAA
AAGCAGCAAACGCTGCCGCTGAAGCCGGACTAAAGAAGGGTCAACAATCCACCGTAGAAA
CAGAGAGCATGCAGTCAAAAGAAACCAGTGGTATTCTTCATTCTCCTTCTACAGAGCGGT
TAAGTCCTCAAAAACGCAACATAGAGGAAGCAATCTTACGCAAGAACGAAACTGAAACCA
AAATGAGTTACCTTCAGCTGAAAAAAGCCATAGTAGCAGAAGTATTGGAAAGAGAATCTG
ATAACACTAGTCCTCTCACCAGCGTAACTCCTCAAGAACCGCGGATACTAAGGAACGAGG
AAGCGGGGAAACTAGAAAGCGGTACAGAGGGAACTCAAGAGACACCGTTCCTTGAGACAC
AAGAAATGTCATTCCAAAGCGGATGGGAAGAGCAAGAAGAGTATGAGGATGAGCAGTCTT
ATTACGGAGACATGAGCTATGACTGGTTTACAGA

Last Updated on 06.12.2018 16:11