Sequences from confirmation amplicon


Confirmation sequences for line 634D03 and hit At4g14400 are shown.
Line ID 634D03
Locus At4g14400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8297280
Primers used for PCR RU79 - 8474, show primer details
   
Sequence Name 51-K027356-022-634-D03C-8409
GenBank Accession LN507382 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K027356-022-634-E01C-8409
GCCATTTACAATTGAATTATCCTGACATGGATCCAATAAAACGAACATCATTAAATTATT
AAAGAGTTTATAAAACGTTGTCCAGCTTCAAAATACCTTCTTAACAGACTTGGTCAGAAC
ATTCTCCACGTGGCGGCAAAAAATGAGGCCTCTCTTACTGCATATATGTTGATGCATGAT
AAAGATACGAAACATCTGGGTGTTGGGCAAGATGTGGACGGGAATACACCTTTGCACCTT
GCCGTCATGAACTGGGACTTTGACTCCATTACTTGTCTTGCTAGCCGTAACCACGAGATA
CTGAAATTACGAAACAAAAGCGGCTTAAGAGCTAGGGATATTGCCGAGTCAGAGGTGAAA
CCCAACTACATCTTTCATGAGGTATTTCTGATTCCGTTTCATACAACACATTTGATCCCT
CATAAATGATTTATAGCTTGACCTCCATATTTTTGGAATTACAGAGGTGGACACTGGCGC
TCTTATTATACGCTATTCACTCAAGTGGTTTCGAATCTGTTAAGTCATTAACAATACAGT
CAGTGCCACTAGACCCTAAGAAAAACAGACATTACGTCAACGCTCTTCTCGTGGTAGCGG
CTCTTGTAGCCACAGTGACGTTTGCTGCAGGCTTTACAATACCAGGTGGTTATATCAGCG
ATAGCAAAAAACCAAACTTGGGCAGGGCAACTTTGGCCACTAACCCAACTCTCTTCTTTT
   
Sequence Name 52-K027356-022-634-D03C-RU79
GenBank Accession LN507694 [GenBank]
Sequencing primer gene specific primer, RU79: 5'-AAGCAGAAAAATGAAGAGAGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K027356-022-634-E01C-RU79
CCAGTTGGTTTTTTGCTTCGCTCATATAACCACCTGGTATTGTAAAGCCCCCTTAAAACG
TCACTGTGGCTACAAGAGCCGCTACCACGACATGAGAGTTGANGTAATGTNTGTTTTTNT
TAGGGTNTAGTGGNANTGANTGTATTGTTAATGANTTAANAGATCCGAAACCACTTGAGT
GAATAGCGTATAATAAGAGCGCCAGTGTCCACCTCTGTAATTCCAAAAATATGGAGGTCA
AGCTATAAATCATTTATGAGGGATCAAATGTGTTGTATGAAACGGAATCAGAAATACCTC
ATGAAAGATGTAGTTGGGTTTCACCTCTGACTCGGCAATATCCCTAGCTCTTAAGCCGCT
TTTGTTTCGTAATTTCAGTATCTCGTGGTTACGGCTAGCAAGACAAGTAATGGAGTCAAA
GTCCCAGTTCATGACGGCAAGGTGCAAAGGTGTATTCCCGTCCACATCTTGCCCAACACC
CAGATGTTTCGTATCTTTATCATGCATCAACATATATGCAGTAAGAGAGGCCTCATTTTT
TGCCGCCACGTGGAGAATGTTCTGACCAAGTCTGTTAAGAAGGTATTTTGAAGCTGGACA
ACGTTTTATAAACTCTTTAATAATTTCGTAATGTTCGTTTTATTGGATCCATGTCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCT
Primers used for PCR NJ58 - 8474, show primer details
   
Sequence Name 76-K036620-022-634-D03C-NJ58
GenBank Accession LN513342 [GenBank]
Sequencing primer gene specific primer, NJ58: 5'-TTTTTGCTATCGCTGATATAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K036620-022-634-D03C-NJ58
GCTGCACAACGTCACTGTGGCTACAGAGCCGCTACCACGAGAAGAGCGTTGACGTAATGT
CTGTTTTTCTTAGGGTCTAGTGGCACTGACTGTATTGTTAATGACTTAACAGATTCGAAA
CCACTTGAGTGAATAGCGTATAATAAGAGCGCCAGTGTCCACCTCTGTAATTCCAAAAAT
ATGGAGGTCAAGCTATAAATCATTTATGAGGGATCAAATGTGTTGTATGAAACGGAATCA
GAAATACCTCATGAAAGATGTAGTTGGGTTTCACCTCTGACTCGGCAATATCCCTAGCTC
TTAAGCCGCTTTTGTTTCGTAATTTCAGTATCTCGTGGTTACGGCTAGCAAGACAAGTAA
TGGAGTCAAAGTCCCAGTTCATGACGGCAAGGTGCAAAGGTGTATTCCCGTCCACATCTT
GCCCAACACCCAGATGTTTCGTATCTTTATCATGCATCAACATATATGCAGTAAGAGAGG
CCTCATTTTTTGCCGCCACGTGGAGAATGTTCTGACCAAGTCTGTTAAGAAGGTATTTTG
AAGCTGGACAACGTTTTATAAACTCTTTAATAATTTCGTAATGTTCGTTTTATTGGATCC
ATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTC
   
Sequence Name 75-K036620-022-634-D03C-8409
GenBank Accession LN513188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K036620-022-634-D03C-8409
CCATTTACAATTGAATATATCCTGACATGGATCCAATAAAACGAACATTACGAAATTATT
AAAGAGTTTATAAAACGTTGTCCAGCTTCAAAATACCTTCTTAACAGACTTGGTCAGAAC
ATTCTCCACGTGGCGGCAAAAAATGAGGCCTCTCTTACTGCATATATGTTGATGCATGAT
AAAGATACGAAACATCTGGGTGTTGGGCAAGATGTGGACGGGAATACACCTTTGCACCTT
GCCGTCATGAACTGGGACTTTGACTCCATTACTTGTCTTGCTAGCCGTAACCACGAGATA
CTGAAATTACGAAACAAAAGCGGCTTAAGAGCTAGGGATATTGCCGAGTCAGAGGTGAAA
CCCAACTACATCTTTCATGAGGTATTTCTGATTCCGTTTCATACAACACATTTGATCCCT
CATAAATGATTTATAGCTTGACCTCCATATTTTTGGAATTACAGAGGTGGACACTGGCGC
TCTTATTATACGCTATTCACTCAAGTGGTTTCGAATCTGTTAAGTCATTAACAATACAGT
CAGTGCCACTAGACCCTAAGAAAAACAGACATTACGTCAACGCTCTTCTCGTGGTAGCGG
CTCTTGTAGCCACAGTGACGTTTGCTGCAGGCTTTACAATACCAGGTGGTTATATCAG

Last Updated on 06.12.2018 16:11