Sequences from confirmation amplicon


Confirmation sequences for line 635A01 and hit At4g22380 are shown.
Line ID 635A01
Locus At4g22380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11812803
Primers used for PCR 28wx - 8474, show primer details
   
Sequence Name 67-K118874-0022-635-A01C-28wx
GenBank Accession LN511913 [GenBank]
Sequencing primer gene specific primer, 28wx: 5'-TGAATCATGTCTTCCTCAAGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118874-0022-635-A01C-28wx
CTATAGCTATGATGAATCATGTCTTCCTCAAGAGTAAATTTGAAGCATACACCAATAATT
ACATAGATACAATGATTCAATTGAAGACCAAAACAGAGTAAACAGAGAAAGTTTCCTGAA
AGAACTCAACAGAGAAAAGTATGTTCAACACAAAATTGCATAAAGCTAAGAATGGCTTCT
TAAGCTTCTAACAACCACCAAAATCTGAGAAACACTAACCTAACGATACACAGCCTCAAA
AATTGCAACCTAATAGCTCTCTATTCTACACGAGTTGAATTAAAATTGCAACCAAATCAT
CGAGAGAGAGAAAGAGATGGTAACCTTATCTTCGGCAAGCAAAGGAAGATGAAGAAGAAT
CTCAAGTGGTTCAGCATCAGCAGCCATAACAACGAACTCAGAGATTCCACGGTTCAATGT
TTTGGTAGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCG
   
Sequence Name 27-K118873-0022-635-A01C-8409
GenBank Accession LN498614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118873-0022-635-A01C-8409
ACATTGAAGCTACCAAAACATTGAACCGTGGAATCTCTGAGTTCGTTGTTATGGCTGCTG
ATGCTGAACCACTTGAGATTCTTCTTCATCTTCCTTTGCTTGCCGAAGATAAGGTTACCA
TCTCTTTCTCTCTCTCGATGATTTGGTTGCAATTTTAATTCAACTCGTGTAGAATAGAGA
GCTATTAGGTTGCAATTTTTGAGGCTGTGTATCGTTAGGTTAGTGTTTCTCAGATTTTGG
TGGTTGTTAGAAGCTTAAGAAGCCATTCTTAGCTTTATGCAATTTTGTGTTGAACATACT
TTTCTCTGTTGAGTTCTTTCAGGAAACTTTCTCTGTTTACTCTGTTTTGGTCTTCAATTG
AATCATTGTATCTATGTAATTATTGGTGTATGCTTCAAATTTACTCTTGAGGAAGACATG
ATTCATCATAGCTATAGTTTCTCCTAGTTTATGTTTAAAAATTGTAGCTTTGAGTTCACT
CTTGAGGAAACCATTGATTCAA

Last Updated on 06.12.2018 16:11