Sequences from confirmation amplicon


Confirmation sequences for line 635D09 and hit At2g35070 are shown.
Line ID 635D09
Locus At2g35070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14784090
Primers used for PCR NG62 - 8474, show primer details
   
Sequence Name 65-K102370-0022-635-D09C-8409
GenBank Accession LN511364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K102370-0022-635-D09C-8409
TCGGACTGACCATTTACTTGCAGAGAGTTTCGAAAAATGCGAGCCGATATTGAAAAGATG
CGAGCGGATATTGCTACAATACAAGCAGAGGAGAGGTATTAAATTTGACTCGCTTTTTAA
TATTTGTTTTTGTCCCATTTAAACTTAGACCTTTCCTTTGAAACAGGTGTGATCACGATA
ACCACATACACAAAGTTGAGCAGGTATAGCTTTTTTTTTCCTAGCTCTATCCATTTATTT
TACACTCGTTATTACTTGAGATTAGTCTTTAGATCGGAAATCAGAAGAAGTAATATATGT
TTTGGGTATTTCAGGGTTTTAACGAGTTAAAAGATGAGTTCGACGCAACAAAATATAAGA
TCGTGAAGTATGCTATATATACACTGATCCCTGTGGCAGCAGCTGAAACTGGTATGCTTT
TCTCGATGATATACTAGCGACATTAT
   
Sequence Name 65-K102371-0022-635-D09C-NG62
GenBank Accession LN511365 [GenBank]
Sequencing primer gene specific primer, NG62: 5'-AAAGATCCGATAACGTCGCTAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K102371-0022-635-D09C-NG62
CCACAGGGATAGTGTATATATAGCATACTTCACGATCTTATATTTTGTTGCGTCGAACTC
ATCTTTTAACTCGTTAAAACCCTGAAATACCCAAAACATATATTACTTCTTCTGATTTCC
GATCTAAAGACTAATCTCAAGTAATAACGAGTGTAAAATAAATGGATAGAGCTAGGAAAA
AAAAAGCTATACCTGCTCAACTTTGTGTATGTGGTTATCGTGATCACACCTGTTTCAAAG
GAAAGGTCTAAGTTTAAATGGGACAAAAACAAATATTAAAAAGCGAGTCAAATTTAATAC
CTCTCCTCTGCTTGTATTGTAGCAATATCCGCTCGCATCTTTTCAATATCGGCTCGCATT
TTTCGAAACTCTTCTGCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGAGGNTCAATAT
Primers used for PCR NG63 - 8474, show primer details
   
Sequence Name 66-K102370-0022-635-D09C-OriL
GenBank Accession LN511571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K102370-0022-635-D09C-OriL
GCCATCAACCGAGTTGATTTATACTCCTATACACAAAAAAAGACAATGAAGAACACTAAT
TAGGTTGAAAAAAGAAACAGATCTTCTAGCATTCCTTAAGTGAGAAAACTAAATGAGTAT
TTATCACGTACGCCATGGAGATAACAACACAAGATATTAACAAAGACATCTATACTTCTG
GACACTTGTCATCAACACAAGATACCTTAACCGTTTCTAAGGATAATTAAGTTCACAACA
CAAGATATACTAGAAAACAGATCTCTACACACGATCCATCATATGTTCCTGACTTCCTGT
AGTAATAGTGAGAATACCAGTACTAATAATTAACGAAAGTTTGGAAAAGAAGAATTGCCT
CGGATTTGGCGATGTCGGCTTTAACCTTCCCCATCACCGACTCAAAGCCACTGGTGATTG
CGCCGGTTAATGCCTCCGCTTGTGTTGAGGGTACGTTGATCGCCTCTAGAGCCTTAACCA
TTCCTAATTTGTCGACATGATT
   
Sequence Name 66-K102371-0022-635-D09C-NG63
GenBank Accession LN511572 [GenBank]
Sequencing primer gene specific primer, NG63: 5'-GAACCATGTCGACAAATTAGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K102371-0022-635-D09C-NG63
CGTACCTCACACAAGCGGAGGCATTAACCGGCGCAATCACCAGTGGCTTTGAGTCGGTGA
TGGGGAAGGTTAAAGCCGACATCGCCAAATCCGAGGCAATTCTTCTTTTCCAAACTTTCG
TTAATTATTAGTACTGGTATTCTCACTATTACTACAGGAAGTCAGGAACATATGATGGAT
CGTGTGTAGAGATCTGTTTTCTAGTATATCTTGTGTTGTGAACTTAATTATCCTTAGAAA
CGGTTAAGGTATCTTGTGTTGATGACAAGTGTCCAGAAGTATAGATGTCTTTGTTAATAT
CTTGTGTTGTTATCTCCATGGCGTACGTGATAAATACTCATTTAGTTTTCTCACTTAAGG
AATGCTAGAAGATCTGTTTCTTTTTTCAACCTAATTAGTGTTCTTCATTGTCTTTTTTTG
TGTATAGGAGTATAAATCAACTCGTGTTGATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATA

Last Updated on 06.12.2018 16:11