Sequences from confirmation amplicon


Confirmation sequences for line 635G07 and hit At5g23660 are shown.
Line ID 635G07
Locus At5g23660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7972819
Primers used for PCR NA35 - 8474, show primer details
   
Sequence Name 77-K101672-0022-635-G07C-8409
GenBank Accession LN513521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K101672-0022-635-G07C-8409
TTTCATTGACATTAAAAACGGCATGTACTCCACACTTTTCGTCTTTATCACCGTTCTCTG
TTTTTTTAATACAAACCTAATTAGTAAAAATCATATTTTCTTCATATATACTTCTGTAGT
AACATTAGCTAGAGACTATTACTACTTACGATAATGCTAAGCGGCGCAGCAAAAACGCAG
ACAGAGAATCCGACACAGATACCTCCAATGATCTTCGCACGTGTGGTTCCTTTTGCCAAG
AATTGGCAGAGGAGGAGAATCAAACAGAACCCTCCAAAGTTCATTAGCAACAAGAGCTTC
ACCGTTAGCATCTGCACCAAAATAATCAATAAATATCACACATACNATAAACATCACATA
TACATATATTTATGTTGGTAATGTTACATACTCGGGCTTTCTTGGATGCGAAGGCAACAA
AGATGGAGATGTATATGGTTTCAATGAAGCAACCAAAGCTGTTGATGGTGACGAGAAGGA
AGACATCTTTCTTCTGAGTAGCGTAGTAGAGCCAAAGCATCGCGCTGAAGAGCGCCACCA
CATAGGGAATAGATTGAAATCCTTCTGTGGTTTTCTTCTTACAAATCCTATA
   
Sequence Name 77-K101679-0022-635-G07C-NA35
GenBank Accession LN513522 [GenBank]
Sequencing primer gene specific primer, NA35: 5'-GGCCAACGTTCTATAGGATTTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K101679-0022-635-G07C-NA35
CATCTATTCCTATGTGGTGGCGCTCTTCAGCGCGATGCTTTGGCTCTACTACGCTACTCA
GAAGAAAGATGTCTTCCTTCTCGTCACCATCAACAGCTTTGGTTGCTTCATTGAAACCAT
ATACATCTCCATCTTTGTTGCCTTCGCATCCAAGAAAGCCCGAGTATGTAACATTACCAA
CATAAATATATGTATATGTGATGTTTATTGTATGTGTGATATTTATTGATTATTTTGGTG
CAGATGCTAACGGTGAAGCTCTTGTTGCTAATGAACTTTGGAGGGTTCTGTTTGATTCTC
CTCCTCTGCCAATTCTTGGCAAAAGGAACCACACGTGCGAAGATCATTGGAGGTATCTGT
GTCGGATTCTCTGTCTGCGTTTTTGCTGCGCCGCTNAGCATTATCGTAAGTAGTAATAGT
CTCTAGCTAATGTTACTACAGAAGTATATATGAAGAAAATATGATTTTTACTAATTAGGT
TTGTATTAAAAAAACAGAGAACGGTGATAAAGACGAAAAGTGTGGAGTACATGCCGTTTT
TAATTGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT
AGATGTCCGCA

Last Updated on 06.12.2018 16:11