Sequences from confirmation amplicon


Confirmation sequences for line 638B11 and hit At5g58900 are shown.
Line ID 638B11
Locus At5g58900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23784193
Primers used for PCR 51hw - 8474, show primer details
   
Sequence Name 54-K112689-0022-638-B11C-51hw
GenBank Accession LN508529 [GenBank]
Sequencing primer gene specific primer, 51hw: 5'-AAAGTTCCGAGATATGTTTCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K112689-0022-638-B11C-51hw
ATAGCCTGAATTGTGACAAATATGGAATCAAATATATCTACATACAATCTTGACCCAAAT
AAATTGTTCTTTGTAGGTTTGATGCGTTATATATGTGATTAGGCAAGATTTTGCAGCTAA
ACAGTGTTGCATAAATCATACTACGATTATGCAATGCATTTGTTCAAACAAATTAGAAAT
AGCCCGATTGTGAGAAATATCTAATCTAAGATATTTTCATAAAGTTATATACAACCACAG
TGTTATACATTTATACTATCAAATTGTCATTAACTAGTAATTTAAATATTGGATATTTTT
CCAATCAATCATGTCACTGATTTATGTTTAACTATTTTACTACCTTGCATGATTAGTGAC
CAACATTTATGTCAATTTCACCACTGACTGAGCTGATTTTTAAGTAGATTTAAATTTCAC
CATTCCGGTATAAAACCATCTAATTCCGGTTTAATGTCAATTCCCAATAAACCGGAAAAG
AACAGAGATTTTTTCTTTCTTTTTTGTGTAAAAGAAGAAACAGAGACTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATTC
   
Sequence Name 22-K112688-0022-638-B11C-8409
GenBank Accession LN496185 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112688-0022-638-B11C-8409
AAAAGAAAGAAAAATCTCTGTTCTTTTCCGGTTTATTGGGAATTGACATTAAACCGGAAT
TAGATGGTTTTATACCGGAATGGTGAAATTTAAATCTACTTAAAAATCAGCTCAGTCAGT
GGTGAAATTGACATAAATGTTGGTCACTAATCATGCAAGGTAGTAAAATAGTTAAACATA
AATCAGTGACATGATTGATTGGAAAAATATCCAATATTTAAATTACTAGTTAATGACAAT
TTGATAGTATAAATGTATAACACTGTGGTTGTATATAACTTTATGAAAATATCTTAGATT
AGATATTTCTCACAATCGGGCTATTTCTAATTTGTTTGAACAAATGCATTGCATAATCGT
AGTATGATTTATGCAACACTGTTTAGCTGCAAAATCTTGCCTAATCACATATATNACGCA
TCAAACCTACAAAGAACAATTTATTTGGGTCAAGATTGTATGTAGATATATTTGATTCCA
TATTTGTCACAATTCAGGCTATTTCTAATGGGTTTGAAGAAATATGGGAAAGGAGATTGG
AGAA

Last Updated on 06.12.2018 16:11