Sequences from confirmation amplicon


Confirmation sequences for line 638G09 and hit At1g07410 are shown.
Line ID 638G09
Locus At1g07410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2276599
Primers used for PCR MN37 - 8474, show primer details
   
Sequence Name 09-K031753-022-638-G09C-8409
GenBank Accession LN490060 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K031753-022-638-G09C-8409
CATTTACAATTAGATTATTCTAACGAGAATTCATTTTCATAACATAAGATCTTCAGATCT
AACAATCACATGCCTGTTCAAATATCAGAGGATGCAAATGCGACGACACAATCTAAGATT
AAACACCAAATTGATCTGGAGGGAAATTGTCTAGATTTAGAGAAAGCGAGAGAAGAGAGA
TGACCTGTAAAGTCCGGGTGGCGAATTCGACGCCAATGGTGGATTTGGATTCGAGACAGA
ACTCGTTTCTGGTGAATCGAGAGAGAATGTTGGATTTACCAACACCGGAATCGCCGATCA
GGACGATCTTGAACAAGTAATCGTACTCATGATCTATTCTATTCGCCATTGCTTCTCAAA
TCCTAGCGTCTGATCAACCT
   
Sequence Name 10-K031753-022-638-G09C-MN37
GenBank Accession LN490535 [GenBank]
Sequencing primer gene specific primer, MN37: 5'-TAGGTTGATCAGACGCTAGGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K031753-022-638-G09C-MN37
ATCATGAGTACGATTACTTGTTCAAGATCGTCCTGATCGGCGATTCCGGTGTTGGTAAAT
CCAACATTCTCTCTCGATTCACCAGAAACGAGTTCTGTCTCGAATCCAAATCCACCATTG
GCGTCGAATTCGCCACCCGGACTTTACAGGTCATCTCTCTTCTCTCGCTTTCTCTAAATC
TAGACAATTTCCCTCCAGATCAATTTGGTGTTTAATCTTAGATTGTGTCGTCGCATTTGC
ATCCTCTGATATTTGAACAGGCATGTGATTGTTAGATCTGAAGATCTTATGTTATGAAAA
TGAATTCTCGTTAGAATAATCTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11